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PMID: 19487668 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 106 ·No. 26 ·2009-06-30 ·Pages 10644-8

Chen JZ, Settembre EC, Aoki ST, Zhang X, Bellamy AR, Dormitzer PR, Harrison SC, Grigorieff N

Abstract

Rotaviruses, major causes of childhood gastroenteritis, are nonenveloped, icosahedral particles with double-strand RNA genomes. By the use of electron cryomicroscopy and single-particle reconstruction, we have visualized a rotavirus particle comprising the inner capsid coated with the trimeric outer-layer protein, VP7, at a resolution (4 A) comparable with that of X-ray crystallography. We have traced the VP7 polypeptide chain, including parts not seen in its X-ray crystal structure. The 3 well-ordered, 30-residue, N-terminal "arms" of each VP7 trimer grip the underlying trimer of VP6, an inner-capsid protein. Structural differences between free and particle-bound VP7 and between free and VP7-coated inner capsids may regulate mRNA transcription and release. The Ca(2+)-stabilized VP7 intratrimer contact region, which presents important neutralizing epitopes, is unaltered upon capsid binding.

MeSH Terms
Antigens, Viral/chemistry,metabolism,ultrastructure Calcium/metabolism Capsid Proteins/chemistry,metabolism,ultrastructure Cryoelectron Microscopy/methods Crystallography, X-Ray Models, Biological Models, Molecular Protein Multimerization Protein Structure, Quaternary Rotavirus/metabolism,ultrastructure Viral Core Proteins/chemistry,metabolism,ultrastructure Virion/metabolism,ultrastructure
Chemicals
Antigens, Viral Capsid Proteins VP1 protein, Rotavirus VP2 protein, Rotavirus VP3 protein, Rotavirus VP7 protein, Rotavirus Viral Core Proteins Calcium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chen James Z
Rosenstiel Basic Medical Research Center, Brandeis University, Waltham, MA 02454, USA.
Settembre Ethan C
Aoki Scott T
Zhang Xing
Bellamy A Richard
Dormitzer Philip R
Harrison Stephen C
Grigorieff Nikolaus
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0904024106
Published
2009-06-30
Epub
2009-00-01
Pages
10644-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2689313
Subset
IM
Grants
NIAID NIH HHS · AI-053174 · United States
NIGMS NIH HHS · P01 GM062580 · United States
NIAID NIH HHS · R01 AI053174 · United States
NCI NIH HHS · R01 CA013202 · United States
NIGMS NIH HHS · GM-62580 · United States
NCI NIH HHS · CA-13202 · United States
Howard Hughes Medical Institute · United States
NCI NIH HHS · R37 CA013202 · United States
Databases
PDB
3GZT, 3GZU, 7RP, VP2, VP3, VP4, VP5, VP6, VP7
Corrections
CommentIn
PMID: 19553214
External Links
PubMed source DOI: 10.1073/pnas.0904024106 PMC Full Text View journal details
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