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PMID: 14963127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of rotavirus cell entry.

Journal of virology ·Vol. 78 ·No. 5 ·2004-03-00 ·Pages 2310-8

Sánchez-San Martín C, López T, Arias CF, López S

Abstract

While recently we have learned much about the viral and cellular proteins involved in the initial attachment of rotaviruses to MA104 cells, the mechanism by which these viruses reach the interior of the cell is poorly understood. For this study, we observed the effects of drugs and of dominant-negative mutants, known to impair clathrin-mediated endocytosis and endocytosis mediated by caveolae, on rotavirus cell infection. Rotaviruses were able to enter cells in the presence of compounds that inhibit clathrin-mediated endocytosis as well as cells overexpressing a dominant-negative form of Eps15, a protein crucial for the assembly of clathrin coats. We also found that rotaviruses infected cells in which caveolar uptake was blocked; treatment with the cholesterol binding agents nystatin and filipin, as well as transfection of cells with dominant-negative caveolin-1 and caveolin-3 mutants, had no effect on rotavirus infection. Interestingly, cells treated with methyl-beta-cyclodextrin, a drug that sequesters cholesterol from membranes, and cells expressing a dominant-negative mutant of the large GTPase dynamin, which is known to function in several membrane scission events, were not infected by rotaviruses, indicating that cholesterol and dynamin play a role in the entry of rotaviruses.

MeSH Terms
Animals Calcium-Binding Proteins/genetics,metabolism Caveolae/metabolism Cell Line Cholera Toxin/metabolism Clathrin/antagonists & inhibitors,metabolism Dynamins/genetics,metabolism Endocytosis Filipin/pharmacology Macaca mulatta Mutation Nystatin/pharmacology Rotavirus/physiology
Chemicals
Calcium-Binding Proteins Clathrin Nystatin Filipin Cholera Toxin Dynamins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sánchez-San Martín Claudia
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, Mexico.
López Tomás
Arias Carlos F
López Susana
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.78.5.2310-2318.2004
Published
2004-03-00
Pages
2310-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC369217
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.78.5.2310-2318.2004 PMC Full Text View journal details
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