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PMID: 10954560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Early steps of polyomavirus entry into cells.

Journal of virology ·Vol. 74 ·No. 18 ·2000-09-00 ·Pages 8582-8

Gilbert JM, Benjamin TL

Abstract

The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data presented here indicate that compounds that block endocytosis of both caveola- and clathrin-derived vesicles have no effect on polyomavirus infectivity. Polyomavirus does not appear to colocalize with either clathrin light chain or caveolin-1 by immunofluorescence microscopy. Additionally, expression of a dominant-negative form of dynamin I has no effect on polyomavirus uptake and infectivity. Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner.

MeSH Terms
3T3 Cells Animals Caveolin 1 Caveolins Cell Line Clathrin/metabolism Coated Vesicles/metabolism Dynamin I Dynamins Endocytosis Fluorescent Antibody Technique, Indirect GTP Phosphohydrolases/metabolism Membrane Proteins/metabolism Mice Polyomavirus/pathogenicity,ultrastructure
Chemicals
Cav1 protein, mouse Caveolin 1 Caveolins Clathrin Membrane Proteins Dynamin I GTP Phosphohydrolases Dynamins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilbert J M
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA. jgilbert@hms.harvard.edu
Benjamin T L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.18.8582-8588.2000
Email
jgilbert@hms.harvard.edu
Published
2000-09-00
Pages
8582-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC116371
Subset
IM
Grants
NCI NIH HHS · T32 CA072320 · United States
NCI NIH HHS · 5T32CA72320 · United States
NCI NIH HHS · P01 CA50661 · United States
NCI NIH HHS · R35 CA44343 · United States
NCI NIH HHS · P01 CA050661 · United States
External Links
PubMed source DOI: 10.1128/jvi.74.18.8582-8588.2000 PMC Full Text View journal details
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