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PMID: 15861131 Published · ppublish English Comparative Study Journal Article

A quasi-atomic model of human adenovirus type 5 capsid.

The EMBO journal ·Vol. 24 ·No. 9 ·2005-05-04 ·Pages 1645-54

Fabry CM, Rosa-Calatrava M, Conway JF, Zubieta C, Cusack S, Ruigrok RW, Schoehn G

Abstract

Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1.

MeSH Terms
Adenoviruses, Human/chemistry,genetics,ultrastructure Bacteriophage PRD1/chemistry Capsid/chemistry,ultrastructure Capsid Proteins/chemistry Cryoelectron Microscopy Models, Molecular Mutation
Chemicals
Capsid Proteins hexon capsid protein, Adenovirus penton protein, adenovirus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fabry Céline M S
Laboratoire de Virologie Moléculaire et Structurale, FRE 2854 CNRS-Université Joseph Fourier, Grenoble Cedex, France.
Rosa-Calatrava Manuel
Conway James F
Zubieta Chloé
Cusack Stephen
Ruigrok Rob W H
Schoehn Guy
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600653
Published
2005-05-04
Epub
2005-00-21
Pages
1645-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1142584
Subset
IM
Databases
PDB
2BLD
External Links
PubMed source DOI: 10.1038/sj.emboj.7600653 PMC Full Text View journal details
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