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PMID: 21159868 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Genetics coupled to quantitative intact proteomics links heritable aphid and endosymbiont protein expression to circulative polerovirus transmission.

Journal of virology ·Vol. 85 ·No. 5 ·2011-03-00 ·Pages 2148-66

Cilia M, Tamborindeguy C, Fish T, Howe K, Thannhauser TW, Gray S

Abstract

Yellow dwarf viruses in the family Luteoviridae, which are the causal agents of yellow dwarf disease in cereal crops, are each transmitted most efficiently by different species of aphids in a circulative manner that requires the virus to interact with a multitude of aphid proteins. Aphid proteins differentially expressed in F2 Schizaphis graminum genotypes segregating for the ability to transmit Cereal yellow dwarf virus-RPV (CYDV-RPV) were identified using two-dimensional difference gel electrophoresis (DIGE) coupled to either matrix-assisted laser desorption ionization-tandem mass spectrometry or online nanoscale liquid chromatography coupled to electrospray tandem mass spectrometry. A total of 50 protein spots, containing aphid proteins and proteins from the aphid's obligate and maternally inherited bacterial endosymbiont, Buchnera, were identified as differentially expressed between transmission-competent and refractive aphids. Surprisingly, in virus transmission-competent F2 genotypes, the isoelectric points of the Buchnera proteins did not match those in the maternal Buchnera proteome as expected, but instead they aligned with the Buchnera proteome of the transmission-competent paternal parent. Among the aphid proteins identified, many were involved in energy metabolism, membrane trafficking, lipid signaling, and the cytoskeleton. At least eight aphid proteins were expressed as heritable, isoelectric point isoform pairs, one derived from each parental lineage. In the F2 genotypes, the expression of aphid protein isoforms derived from the competent parental lineage aligned with the virus transmission phenotype with high precision. Thus, these isoforms are candidate biomarkers for CYDV-RPV transmission in S. graminum. Our combined genetic and DIGE approach also made it possible to predict where several of the proteins may be expressed in refractive aphids with different barriers to transmission. Twelve proteins were predicted to act in the hindgut of the aphid, while six proteins were predicted to be associated with the accessory salivary glands or hemolymph. Knowledge of the proteins that regulate virus transmission and their predicted locations will aid in understanding the biochemical mechanisms regulating circulative virus transmission in aphids, as well as in identifying new targets to block transmission.

MeSH Terms
Animals Aphids/genetics,microbiology,physiology,virology Bacterial Proteins/chemistry,genetics,metabolism Buchnera/chemistry,genetics,physiology Edible Grain/virology Gene Expression Insect Proteins/chemistry,genetics,metabolism Luteoviridae/physiology Molecular Sequence Data Plant Diseases/virology Proteomics Symbiosis Two-Dimensional Difference Gel Electrophoresis
Chemicals
Bacterial Proteins Insect Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cilia M
Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA.
Tamborindeguy C
Fish T
Howe K
Thannhauser T W
Gray S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.01504-10
Published
2011-03-00
Epub
2010-00-15
Pages
2148-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC3067806
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.01504-10 PMC Full Text View journal details
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