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PMID: 22215806 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

A decade of 3C technologies: insights into nuclear organization.

Genes & development ·Vol. 26 ·No. 1 ·2012-01-01 ·Pages 11-24

de Wit E, de Laat W

Abstract

Over the past 10 years, the development of chromosome conformation capture (3C) technology and the subsequent genomic variants thereof have enabled the analysis of nuclear organization at an unprecedented resolution and throughput. The technology relies on the original and, in hindsight, remarkably simple idea that digestion and religation of fixed chromatin in cells, followed by the quantification of ligation junctions, allows for the determination of DNA contact frequencies and insight into chromosome topology. Here we evaluate and compare the current 3C-based methods (including 4C [chromosome conformation capture-on-chip], 5C [chromosome conformation capture carbon copy], HiC, and ChIA-PET), summarize their contribution to our current understanding of genome structure, and discuss how shape influences genome function.

MeSH Terms
Animals Cell Nucleus Chromosomes/chemistry Genome Humans Imaging, Three-Dimensional Molecular Biology/methods,trends Molecular Conformation Sequence Analysis, DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
de Wit Elzo
Hubrecht Institute-KNAW, University Medical Center Utrecht, CT Utrecht, The Netherlands.
de Laat Wouter
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.179804.111
Published
2012-01-01
Pages
11-24
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC3258961
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.179804.111 PMC Full Text View journal details
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