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PMID: 16951251 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CTCF mediates long-range chromatin looping and local histone modification in the beta-globin locus.

Genes & development ·Vol. 20 ·No. 17 ·2006-09-01 ·Pages 2349-54

Splinter E, Heath H, Kooren J, Palstra RJ, Klous P, Grosveld F, Galjart N, de Laat W

Abstract

CTCF (CCCTC-binding factor) binds sites around the mouse beta-globin locus that spatially cluster in the erythroid cell nucleus. We show that both conditional deletion of CTCF and targeted disruption of a DNA-binding site destabilize these long-range interactions and cause local loss of histone acetylation and gain of histone methylation, apparently without affecting transcription at the locus. Our data demonstrate that CTCF is directly involved in chromatin architecture and regulates local balance between active and repressive chromatin marks. We postulate that throughout the genome, relative position and stability of CTCF-mediated loops determine their effect on enhancer-promoter interactions, with gene insulation as one possible outcome.

MeSH Terms
Animals CCCTC-Binding Factor Cell Line Cells, Cultured Chromatin/chemistry,metabolism DNA-Binding Proteins/deficiency,genetics,physiology Erythroid Cells/metabolism Fetus Genetic Markers Globins/biosynthesis,genetics,metabolism Histones/metabolism Mice Mice, Knockout Repressor Proteins/genetics,physiology
Chemicals
CCCTC-Binding Factor Chromatin Ctcf protein, mouse DNA-Binding Proteins Genetic Markers Histones Repressor Proteins Globins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Splinter Erik
Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands.
Heath Helen
Kooren Jurgen
Palstra Robert-Jan
Klous Petra
Grosveld Frank
Galjart Niels
de Laat Wouter
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.399506
Published
2006-09-01
Pages
2349-54
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1560409
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.399506 PMC Full Text View journal details
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