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PMID: 14759373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species.

Molecular cell ·Vol. 13 ·No. 2 ·2004-01-30 ·Pages 291-8

Yusufzai TM, Tagami H, Nakatani Y, Felsenfeld G

Abstract

Insulators can block an enhancer of one gene from activating a promoter on another nearby gene. Almost all described vertebrate insulators require binding of the regulatory protein CTCF for their activity. We show that CTCF copurifies with the nucleolar protein nucleophosmin and both are present at insulator sites in vivo. Furthermore, exogenous insulator sequences are tethered to the nucleolus in a CTCF-dependent manner. These interactions, quite different from those of the gypsy insulator element in Drosophila, may generate similar loop structures, suggesting a common theme and model for enhancer-blocking insulator action.

MeSH Terms

Animals Binding Sites CCCTC-Binding Factor Cell Nucleus/metabolism Chickens Chromatin/chemistry,metabolism DNA, Complementary/metabolism DNA-Binding Proteins/metabolism,physiology Dimerization HeLa Cells Humans In Situ Hybridization, Fluorescence K562 Cells Mass Spectrometry Models, Biological Nuclear Proteins/chemistry,metabolism Nucleophosmin Precipitin Tests Protein Binding Repressor Proteins/metabolism,physiology Silver Staining Species Specificity

Chemicals

CCCTC-Binding Factor CTCF protein, human Chromatin DNA, Complementary DNA-Binding Proteins NPM1 protein, human Nuclear Proteins Repressor Proteins Nucleophosmin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yusufzai Timur M

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Tagami Hideaki

Nakatani Yoshihiro

Felsenfeld Gary

Article Info

Journal

Molecular cell

Abbr.

Mol Cell

ISSN

1097-2765

DOI

10.1016/s1097-2765(04)00029-2

Published

2004-01-30

Pages

291-8

Language

English

Region

United States

NLM ID

9802571

Subset

External Links

PubMed source DOI: 10.1016/s1097-2765(04)00029-2 View journal details

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CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species.

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