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PMID: 2164607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

N myristoylation of the spleen necrosis virus matrix protein is required for correct association of the Gag polyprotein with intracellular membranes and for particle formation.

Journal of virology ·Vol. 64 ·No. 8 ·1990-08-00 ·Pages 3995-4001

Weaver TA, Panganiban AT

Abstract

To determine whether myristoylation is required for spleen necrosis virus replication, we constructed a substitution mutation in the gag gene that alters the putative myristate acceptor glycine residue. This single amino acid change was lethal for virus replication, resulted in aberrant proteolytic processing, and interrupted virion assembly and the release of virus from cells. Immunofluorescence analysis indicated that the amount of Gag polyprotein at the cell periphery and in Golgi-associated vesicles is severely reduced in the myristoylation mutant, indicating that correct intracellular targeting is affected by a lack of myristoylation. Coexpression of wild-type Gag polyprotein did not complement and rescue the replication-defective phenotype of the myristoylation mutant. Thus, it appears that the nonmyristoylated polyproteins are incapable of interacting with their myristoylated counterparts to form biologically active particles.

MeSH Terms
Animals Cell Line Codon/genetics DNA, Viral/genetics Gene Products, gag/genetics,metabolism Genes, gag Intracellular Membranes/metabolism Mutation Myristic Acid Myristic Acids/metabolism Protein Binding Protein Processing, Post-Translational Retroviridae/genetics,metabolism,physiology Transfection Viral Matrix Proteins/genetics,metabolism Virus Replication
Chemicals
Codon DNA, Viral Gene Products, gag Myristic Acids Viral Matrix Proteins Myristic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weaver T A
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Panganiban A T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.8.3995-4001.1990
Published
1990-08-00
Pages
3995-4001
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249699
Subset
IM
Grants
NCI NIH HHS · CA22443-10 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.8.3995-4001.1990 PMC Full Text View journal details
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