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PMID: 2186174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Spleen necrosis virus gag polyprotein is necessary for particle assembly and release but not for proteolytic processing.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 2642-52

Weaver TA, Talbot KJ, Panganiban AT

Abstract

The nature of spleen necrosis virus pol gene expression and the role of gag and gag-pol polyproteins in virion assembly was investigated. The DNA sequence of the gag-pol junction revealed that the two genes occupy the same open reading frame but are separated by an in-frame amber stop codon. Biochemical analysis of gag-pol translational readthrough in vitro and in Escherichia coli suggests that, in a manner similar to that in other mammalian type C retroviruses, amber stop codon suppression is required for pol gene expression. Removal of the gag stop codon had little or no effect on synthesis or cleavage of the polyprotein but interrupted particle assembly. This block could be overcome by complementation with wild-type gag protein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Escherichia coli/genetics Gene Products, gag/genetics,metabolism Gene Products, pol/genetics Genes, gag Genes, pol Molecular Sequence Data Molecular Weight Mutation Oligonucleotide Probes Plasmids Protein Processing, Post-Translational Proviruses/genetics,metabolism Retroviridae/genetics,metabolism
Chemicals
Gene Products, gag Gene Products, pol Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Weaver T A
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Talbot K J
Panganiban A T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.2642-2652.1990
Published
1990-06-00
Pages
2642-52
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249442
Subset
IM
Grants
NCI NIH HHS · P0-1-CA22443-10 · United States
Databases
GENBANK
M54993
External Links
PubMed source DOI: 10.1128/JVI.64.6.2642-2652.1990 PMC Full Text View journal details
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