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PMID: 2157967 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum contains three promoters specific for growth, aggregation, and late development.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 1921-30

Faure M, Franke J, Hall AL, Podgorski GJ, Kessin RH

Abstract

The cyclic nucleotide phosphodiesterase (phosphodiesterase) plays essential roles throughout the development of Dictyostelium discoideum. It is crucial to cellular aggregation and to postaggregation morphogenesis. The phosphodiesterase gene is transcribed into three mRNAs, containing the same coding sequence connected to different 5' untranslated sequences, that accumulate at different times during the life cycle. A 1.9-kilobase (kb) mRNA is specific for growth, a 2.4-kb mRNA is specific for aggregation, and a 2.2-kb mRNA is specific for late development and is only expressed in prestalk cells. Hybridization of RNA isolated from cells at various stages of development with different upstream regions of the gene indicated separate promoters for each of the three mRNAs. The existence of specific promoters was confirmed by fusing the three putative promoter regions to the chloramphenicol acetyltransferase reporter gene, and the analysis of transformants containing these constructs. The three promoters are scattered within a 4.1-kilobase pair (kbp) region upstream of the initiation codon. The late promoter is proximal to the coding sequence, the growth-specific promoter has an initiation site that is 1.9 kbp upstream of the ATG codon, and the aggregation-specific promoter has an initiation site 3 kbp upstream.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Aggregation Cell Division Dictyostelium/cytology,genetics,growth & development Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Phosphoric Diester Hydrolases/genetics Polymerase Chain Reaction Promoter Regions, Genetic RNA, Fungal/genetics RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Fungal RNA, Messenger Phosphoric Diester Hydrolases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Faure M
Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Franke J
Hall A L
Podgorski G J
Kessin R H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.1921-1930.1990
Published
1990-05-00
Pages
1921-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360538
Subset
IM
Grants
NIGMS NIH HHS · GM33136 · United States
Databases
GENBANK
M23449
External Links
PubMed source DOI: 10.1128/mcb.10.5.1921-1930.1990 PMC Full Text View journal details
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