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PMID: 2843545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein.

The Journal of cell biology ·Vol. 107 ·No. 2 ·1988-08-00 ·Pages 521-8

Kesbeke F, Snaar-Jagalska BE, Van Haastert PJ

Abstract

Transmembrane signal transduction was investigated in four Dictyostelium discoideum mutants that belong to the fgd A complementation group. The results show the following. (a) Cell surface cAMP receptors are present in fgd A mutants, but cAMP does not induce any of the intracellular responses, including the activation of adenylate or guanylate cyclase and chemotaxis. (b) cAMP induces down-regulation and the covalent modification (presumably phosphorylation) of the cAMP receptor. (c) The inhibitory effects of GTP gamma S and GDP beta S on cAMP binding are reduced; the stimulatory effect of cAMP on GTP gamma S binding is lost in fgd A mutants. (d) Basal high-affinity GTPase activity is reduced 40% and the stimulatory effect of cAMP is decreased from 40% in wild type to 30% in fgd A. (e) GTP-mediated stimulation and inhibition of adenylate cyclase is normal in mutant membranes. The results suggest a defective interaction between cell surface cAMP receptors and a specific G-protein in fgd A mutants. This interaction appears to be essential for nearly all signal transduction pathways in Dictyostelium discoideum.

MeSH Terms

Adenylyl Cyclases/metabolism Binding, Competitive Chemotaxis Cyclic AMP/metabolism Dictyostelium/genetics,metabolism Electrophoresis, Polyacrylamide Gel GTP Phosphohydrolases/metabolism GTP-Binding Proteins/genetics,metabolism Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Diphosphate/analogs & derivatives,metabolism Guanosine Triphosphate/analogs & derivatives,metabolism Immunoassay Kinetics Mutation Receptors, Cyclic AMP/genetics,metabolism Thionucleotides/metabolism

Chemicals

Receptors, Cyclic AMP Thionucleotides Guanosine Diphosphate Guanosine 5'-O-(3-Thiotriphosphate) guanosine 5'-O-(2-thiodiphosphate) Guanosine Triphosphate Cyclic AMP GTP Phosphohydrolases GTP-Binding Proteins Adenylyl Cyclases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kesbeke F

Department of Biology, University of Leiden, The Netherlands.

Snaar-Jagalska B E

Van Haastert P J

References (16)

16 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.107.2.521

Published

1988-08-00

Pages

521-8

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2115228

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1083/jcb.107.2.521 PMC Full Text View journal details

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Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein.

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