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PMID: 6828377 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.

Nucleic acids research ·Vol. 11 ·No. 2 ·1983-01-25 ·Pages 541-52

Kimmel AR, Firtel RA

Abstract

We have compared the sequences which lie 5' to the coding regions of 15 Dictyostelium genes transcribed by RNA polymerase II. These sequences are extremely (approximately 90%) A + T rich and contain extensive homopolymeric regions. Like most eukaryotic genes, those in Dictyostelium possess a TATA or Goldberg-Hogness Box approximately 25-35bp 5' to the site of transcription initiation. In addition, each gene contains an oligo (dT) stretch between the TATA Box and mRNA start site; this oligo (dT) sequence is, thus far, unique to Dictyostelium. We suggest that the TATA-oligo (dT) structure is an essential component of the Dictyostelium promoter. The general sequence structure of coding, non-coding and untranscribed flanking regions in Dictyostelium is also discussed.

MeSH Terms
Base Sequence Dictyostelium/genetics Fungal Proteins/genetics Genes RNA Polymerase II/metabolism Species Specificity Transcription, Genetic
Chemicals
Fungal Proteins RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kimmel A R
Firtel R A
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.2.541
Published
1983-01-25
Pages
541-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325732
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.2.541 PMC Full Text View journal details
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