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PMID: 2601692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4653-9

May T, Kern H, Müller-Taubenberger A, Nellen W

Abstract

Using a new promoter analysis transformation vector for Dictyostelium discoideum (PAV-CAT), we have defined cis-acting elements in the promoter of the cyclic AMP-induced early-expressed gene A11H2, which encodes an alpha-fucosidase-related protein (A. Müller-Taubenberger, M. Westphal, A. Noegel, and G. Gerisch, FEBS Lett. 246:185-192, 1989). Sequences responsible for developmentally regulated gene induction could be separated from the basal promoter that conferred low levels of transcriptional activity. By gel shift experiments, we present evidence that the cis-acting element is the target of a trans-acting factor that by itself is subject to developmental regulation.

MeSH Terms
Base Sequence Blotting, Northern Chromatography, Thin Layer Cloning, Molecular Dictyostelium/genetics Enzyme Induction Gene Expression Regulation, Fungal Molecular Sequence Data Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Time Factors Transcription, Genetic Transcriptional Activation Transformation, Genetic alpha-L-Fucosidase/genetics
Chemicals
alpha-L-Fucosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
May T
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Kern H
Müller-Taubenberger A
Nellen W
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4653-4659.1989
Published
1989-11-00
Pages
4653-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363611
Subset
IM
Databases
GENBANK
M30465
External Links
PubMed source DOI: 10.1128/mcb.9.11.4653-4659.1989 PMC Full Text View journal details
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