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PMID: 3816763 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulatory sequences in the promoter of the Dictyostelium Actin 6 gene.

The EMBO journal ·Vol. 5 ·No. 12 ·1986-12-01 ·Pages 3367-72

Nellen W, Silan C, Saur U, Firtel RA

Abstract

The promoter region of the developmentally regulated Actin 6 gene of Dictyostelium has been dissected by a series of deletions. Functional analysis of the deletions in Dictyostelium transformants revealed two short regulatory sequences: a positive upstream element (PUE) between -599 and -572 which increases transcription by a factor of 10 but does not affect the developmental pattern of expression and an upstream activator sequence (UAS) between -249 and -215 which is essential for transcription and proper developmental regulation. The UAS partially coincides with a conserved sequence with dyad symmetry found upstream of several Dictyostelium actin genes (Romans and Firtel, 1985a).

MeSH Terms
Actins/genetics Base Sequence Genes Genes, Fungal Genes, Regulator Promoter Regions, Genetic Transcription, Genetic
Chemicals
Actins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nellen W
Silan C
Saur U
Firtel R A
References (25)
25 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Spatial and temporal regulation of a foreign gene by a prestalk-specific promoter in transformed Dictyostelium discoideum.
    Mol Cell Biol. 1986 Mar;6(3):811-20 PMID: 3022131
  3. Multiple, heterogeneous actin genes in Dictyostelium.
    Cell. 1978 Nov;15(3):789-800 PMID: 728991
  4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  5. Unusual nucleotide sequences at the 5' end of actin genes in Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6206-10 PMID: 293714
  6. Isolation of developmentally regulated genes from Dictyostelium.
    Dev Biol. 1980 Oct;79(2):409-18 PMID: 7429011
  7. Differential expression and 5' end mapping of actin genes in Dictyostelium.
    Cell. 1981 Jun;24(3):799-807 PMID: 6894715
  8. Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.
    Nucleic Acids Res. 1983 Jan 25;11(2):541-52 PMID: 6828377
  9. Cell-type-specific actin mRNA populations in dictyostelium discoideum.
    Cell. 1982 Dec;31(2 Pt 1):375-82 PMID: 6297763
  10. Evidence for sub-families of actin genes in Dictyostelium as determined by comparisons of 3' end sequences.
    J Mol Biol. 1981 Oct 5;151(4):593-606 PMID: 6276562
  11. Induction and modulation of cell-type-specific gene expression in Dictyostelium.
    Cell. 1983 Mar;32(3):763-71 PMID: 6299575
  12. Regulation of dictyostelium discoideum mRNAs specific for prespore or prestalk cells.
    Cell. 1983 Apr;32(4):1139-48 PMID: 6301681
  13. Genomic instability and mobile genetic elements in regions surrounding two discoidin I genes of Dictyostelium discoideum.
    Mol Cell Biol. 1984 Apr;4(4):671-80 PMID: 6325889
  14. Developmental regulation of a Dictyostelium gene encoding a protein homologous to mammalian ras protein.
    Cell. 1984 Nov;39(1):141-8 PMID: 6091907
  15. Cloning and expression of a cDNA that comprises part of the gene coding for a spore coat protein of Dictyostelium discoideum.
    Mol Cell Biol. 1984 Nov;4(11):2273-8 PMID: 6549045
  16. DNA-mediated transformation in Dictyostelium discoideum: regulated expression of an actin gene fusion.
    Mol Cell Biol. 1984 Dec;4(12):2890-8 PMID: 6098825
  17. A developmentally regulated cysteine proteinase in Dictyostelium discoideum.
    EMBO J. 1985 Apr;4(4):999-1006 PMID: 2990918
  18. Organization of the Dictyostelium discoideum actin multigene family. Flanking sequences show subfamily homologies and unusual dyad symmetries.
    J Mol Biol. 1985 Jun 5;183(3):311-26 PMID: 2991530
  19. Quantitative in situ gel electrophoretic assay for neomycin phosphotransferase activity in mammalian cell lysates.
    Anal Biochem. 1985 Jul;148(1):101-4 PMID: 2994516
  20. Regulated expression of ras gene constructs in Dictyostelium transformants.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):7005-9 PMID: 3863137
  21. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium.
    Cell. 1985 Dec;43(3 Pt 2):633-41 PMID: 4075402
  22. Gene-specific expression of the actin multigene family of Dictyostelium discoideum.
    J Mol Biol. 1985 Nov 20;186(2):337-55 PMID: 4087297
  23. High-copy-number transformants and co-transformation in Dictyostelium.
    Gene. 1985;39(2-3):155-63 PMID: 4092928
  24. Early Dictyostelium development: control mechanisms bypassed by sequential mutagenesis.
    Cold Spring Harb Symp Quant Biol. 1985;50:813-22 PMID: 2420519
  25. Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genes.
    Cell. 1978 Nov;15(3):763-78 PMID: 728989
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-01
Pages
3367-72
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167335
Subset
IM
Grants
NIGMS NIH HHS · GM30693 · United States
External Links
PubMed source PMC Full Text View journal details
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