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PMID: 2155425 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Murine interleukin 2 receptor beta chain: dysregulated gene expression in lymphoma line EL-4 caused by a promoter insertion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 5 ·1990-03-00 ·Pages 1806-10

Kono T, Doi T, Yamada G, Hatakeyama M, Minamoto S, Tsudo M, Miyasaka M, Miyata T, Taniguchi T

Abstract

The functional, high-affinity interleukin 2 receptor (IL-2R) consists of at least two receptor components, IL-2R alpha (p55) and IL-2R beta (p70-75). The cDNA encoding the murine IL-2R beta has been isolated by using the previously cloned cDNA for human IL-2R beta as a probe. Analysis of the cDNA revealed that the murine IL-2R beta shows a marked homology with the human IL-2R beta and that it is also structurally related to other cytokine receptors such as erythropoietin receptor. The cDNA-directed murine IL-2R beta formed high-affinity IL-2R in conjunction with the endogenous IL-2R alpha in a murine pro-B-cell line and could transduce IL-2-induced growth signal. In mouse lymphoma line EL-4, the IL-2R beta gene was found to be rearranged by the insertion of the long terminal repeat sequence of an intracisternal A particle, giving rise to constitutive expression of the IL-2R beta mRNA.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cloning, Molecular DNA Transposable Elements DNA, Neoplasm/genetics Gene Expression Regulation Gene Library Lymphoma Macromolecular Substances Mice Molecular Sequence Data Promoter Regions, Genetic Receptors, Interleukin-2/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Neoplasm Macromolecular Substances Receptors, Interleukin-2
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kono T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Doi T
Yamada G
Hatakeyama M
Minamoto S
Tsudo M
Miyasaka M
Miyata T
Taniguchi T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.5.1806
Published
1990-03-00
Pages
1806-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53572
Subset
IM
Databases
GENBANK
M28052
External Links
PubMed source DOI: 10.1073/pnas.87.5.1806 PMC Full Text View journal details
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