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PMID: 2467293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 6 ·1989-03-00 ·Pages 1982-6

Tsudo M, Kitamura F, Miyasaka M

Abstract

The human high-affinity receptor for interleukin 2 (IL-2) has been proposed as being a membrane complex composed of at least two distinct polypeptide chains: p55 (alpha chain), recognized by the anti-Tac monoclonal antibody (mAb), and p75 (beta chain), both of which are capable of binding IL-2. Whereas the alpha chain itself has been shown to be nonfunctional, the beta chain appears to be pivotal in the IL-2 signal transduction, although the beta chain is otherwise poorly characterized. Three beta chain-specific mAbs, designated Mik-beta 1, -beta 2, and -beta 3, were developed. Mik-beta 1 and -beta 2 completely inhibited the IL-2 binding to the beta chain, whereas Mik-beta 3 immunoprecipitated the beta chain crosslinked with 125I-labeled IL-2. The beta chain immunoprecipitated by these mAbs was revealed to have a Mr of 68,000-72,000. High-affinity IL-2 binding was completely abolished by Mik-beta 1. Although IL-2-dependent T-cell growth at high IL-2 concentrations was not inhibited by the anti-Tac, it was almost completely inhibited by Mik-beta 1 in the presence of the anti-Tac. These results clearly indicate that the beta chain is an indispensable component to the high-affinity IL-2 receptor and is responsible for the IL-2 signal transduction. The beta chain was found to be constitutively expressed without the alpha chain on the surface of peripheral blood Leu-19+ natural killer cells.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Cell Line Electrophoresis, Polyacrylamide Gel Epitopes/immunology Flow Cytometry Humans Immunosorbent Techniques Interleukin-2/metabolism,pharmacology Killer Cells, Natural Lymphocyte Activation Macromolecular Substances Mice Mice, Inbred BALB C Molecular Weight Receptors, Interleukin-2/analysis,immunology Signal Transduction
Chemicals
Antibodies, Monoclonal Epitopes Interleukin-2 Macromolecular Substances Receptors, Interleukin-2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsudo M
Department of Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.
Kitamura F
Miyasaka M
References (29)
29 references, click to expand
  1. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  2. The IL-2 receptor beta chain (p70): role in mediating signals for LAK, NK, and proliferative activities.
    Science. 1987 Oct 2;238(4823):75-8 PMID: 3116668
  3. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor.
    Nature. 1982 Nov 18;300(5889):267-9 PMID: 6815536
  4. Effects of natural and recombinant IL 2 on regulation of IFN gamma production and natural killer activity: lack of involvement of the Tac antigen for these immunoregulatory effects.
    J Immunol. 1984 Aug;133(2):779-83 PMID: 6203980
  5. Response of resting human peripheral blood natural killer cells to interleukin 2.
    J Exp Med. 1984 Oct 1;160(4):1147-69 PMID: 6434688
  6. Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.
    Nature. 1984 Oct 18-24;311(5987):626-31 PMID: 6090948
  7. Molecular cloning of cDNA encoding human interleukin-2 receptor.
    Nature. 1984 Oct 18-24;311(5987):631-5 PMID: 6090949
  8. Recombinant interleukin 2 enhanced natural killer cell-mediated cytotoxicity in human lymphocyte subpopulations expressing the Leu 7 and Leu 11 antigens.
    J Immunol. 1985 Feb;134(2):794-801 PMID: 3871216
  9. TCGF (IL 2)-receptor inducing factor(s). I. Regulation of IL 2 receptor on a natural killer-like cell line (YT cells).
    J Immunol. 1985 Mar;134(3):1623-30 PMID: 2578514
  10. Stable expression of cDNA encoding the human interleukin 2 receptor in eukaryotic cells.
    J Exp Med. 1985 Jul 1;162(1):363-8 PMID: 2989411
  11. Expression of functional interleukin-2 receptors in human light chain/Tac transgenic mice.
    Nature. 1988 Jan 21;331(6153):267-9 PMID: 2827038
  12. The human interleukin 2 receptor beta chain (p70). Direct identification, partial purification, and patterns of expression on peripheral blood mononuclear cells.
    J Exp Med. 1988 Mar 1;167(3):1265-70 PMID: 2832509
  13. Interleukin-2: inception, impact, and implications.
    Science. 1988 May 27;240(4856):1169-76 PMID: 3131876
  14. Direct identification of the murine IL-2 receptor p55-p75 heterodimer in the absence of IL-2.
    J Immunol. 1988 Jul 15;141(2):476-82 PMID: 3133409
  15. The human intermediate-affinity interleukin 2 receptor consists of two distinct, partially homologous glycoproteins.
    Eur J Immunol. 1988 Jul;18(7):1051-7 PMID: 3136023
  16. Internalization of interleukin 2 is mediated by the beta chain of the high-affinity interleukin 2 receptor.
    J Exp Med. 1987 Apr 1;165(4):1201-6 PMID: 3031195
  17. A second human interleukin-2 binding protein that may be a component of high-affinity interleukin-2 receptors.
    Nature. 1987 Jun 11-17;327(6122):518-22 PMID: 3108674
  18. Contribution of a p75 interleukin 2 binding peptide to a high-affinity interleukin 2 receptor complex.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4215-8 PMID: 3108887
  19. The p75 peptide is the receptor for interleukin 2 expressed on large granular lymphocytes and is responsible for the interleukin 2 activation of these cells.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5394-8 PMID: 3110786
  20. Direct activation of human resting T cells by IL 2: the role of an IL 2 receptor distinct from the Tac protein.
    J Immunol. 1987 Sep 1;139(5):1550-6 PMID: 3040856
  21. Establishment of an interleukin 2-dependent human T cell line from a patient with T cell chronic lymphocytic leukemia who is not infected with human T cell leukemia/lymphoma virus.
    Blood. 1987 Oct;70(4):1069-72 PMID: 3115332
  22. The interleukin 2 receptor. Functional consequences of its bimolecular structure.
    J Exp Med. 1987 Oct 1;166(4):1055-69 PMID: 3116143
  23. Contrasting interleukin 2 binding properties of the alpha (p55) and beta (p70) protein subunits of the human high-affinity interleukin 2 receptor.
    J Exp Med. 1987 Oct 1;166(4):1156-61 PMID: 3116145
  24. The structure, function, and expression of interleukin-2 receptors on normal and malignant lymphocytes.
    Science. 1986 May 9;232(4751):727-32 PMID: 3008337
  25. Novel interleukin-2 receptor subunit detected by cross-linking under high-affinity conditions.
    Science. 1986 Nov 14;234(4778):859-63 PMID: 3095922
  26. Molecular basis for two different affinity states of the interleukin 2 receptor: affinity conversion model.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9026-9 PMID: 3097640
  27. Interleukin 2 high-affinity receptor expression requires two distinct binding proteins.
    J Exp Med. 1987 Jan 1;165(1):223-38 PMID: 3098894
  28. Demonstration of a non-Tac peptide that binds interleukin 2: a potential participant in a multichain interleukin 2 receptor complex.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9694-8 PMID: 3099289
  29. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells.
    J Immunol. 1981 Apr;126(4):1393-7 PMID: 6970774
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.6.1982
Published
1989-03-00
Pages
1982-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286829
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.6.1982 PMC Full Text View journal details
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