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PMID: 2143197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of the v-mos oncogene on Xenopus development: meiotic induction in oocytes and mitotic arrest in cleaving embryos.

The Journal of cell biology ·Vol. 111 ·No. 2 ·1990-08-00 ·Pages 533-41

Freeman RS, Kanki JP, Ballantyne SM, Pickham KM, Donoghue DJ

Abstract

Previous work has demonstrated that the Xenopus protooncogene mosxe can induce the maturation of prophase-arrested Xenopus oocytes. Recently, we showed that mosxe can transform murine NIH3T3 fibroblasts, although it exhibited only 1-2% of the transforming activity of the v-mos oncogene. In this study we have investigated the ability of the v-mos protein to substitute for the mosxe protein in stimulating Xenopus oocytes to complete meiosis. Microinjection of in vitro synthesized RNAs encoding either the mosxe or v-mos proteins stimulates resting oocytes to undergo germinal vesicle breakdown. Microinjection of an antisense oligonucleotide spanning the initiation codon of the mosxe gene blocked progesterone-induced oocyte maturation. When oocytes were microinjected first with the mosxe antisense oligonucleotide, and subsequently with in vitro synthesized v-mos RNA, meiotic maturation was rescued as evidenced by germinal vesicle breakdown. The v-mos protein exhibited in vitro kinase activity when recovered by immunoprecipitation from either microinjected Xenopus oocytes or transfected monkey COS-1 cells; however, in parallel experiments, we were unable to detect in vitro kinase activity associated with the mosxe protein. Microinjection of in vitro synthesized v-mos RNA into cleaving Xenopus embryos resulted in mitotic arrest, demonstrating that the v-mos protein can function like the mosxe protein as a component of cytostatic factor. These results exemplify the apparently conflicting effects of the v-mos protein, namely, its ability to induce maturation of oocytes, its ability to arrest mitotic cleavage of Xenopus embryo, and its ability to transform mammalian fibroblasts.

MeSH Terms
Animals Cell Division Cell Line Embryo, Nonmammalian/cytology Female Gene Expression Meiosis Mitosis Oncogene Proteins v-mos Oncogenes Oocytes/cytology,enzymology Protein-Tyrosine Kinases/metabolism Retroviridae Proteins, Oncogenic/genetics,metabolism Transfection Xenopus
Chemicals
Oncogene Proteins v-mos Retroviridae Proteins, Oncogenic Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Freeman R S
Department of Chemistry, University of California at San Diego, La Jolla 92093.
Kanki J P
Ballantyne S M
Pickham K M
Donoghue D J
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55 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.2.533
Published
1990-08-00
Pages
533-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116195
Subset
IM
Grants
NCI NIH HHS · CA 09523 · United States
NCI NIH HHS · CA 34456 · United States
NIGMS NIH HHS · GM 07313 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.2.533 PMC Full Text View journal details
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