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PMID: 2476810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 7038-42

O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM

Abstract

Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucleus after meiosis I, and cleavage to two cells. Therefore, c-mos is required for meiosis II during murine oocyte maturation.

MeSH Terms
Animals Cells, Cultured Chromatin/ultrastructure Female Meiosis/drug effects Mice Mice, Inbred Strains Microinjections Oligoribonucleotides/pharmacology Oocytes/cytology,drug effects Protein Biosynthesis Proteins/isolation & purification Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-mos Proto-Oncogenes RNA/genetics RNA, Antisense RNA, Messenger/antagonists & inhibitors
Chemicals
Chromatin Oligoribonucleotides Proteins Proto-Oncogene Proteins RNA, Antisense RNA, Messenger RNA Proto-Oncogene Proteins c-mos
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Keefe S J
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Wolfes H
Kiessling A A
Cooper G M
References (35)
35 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Cyclin synthesis, modification and destruction during meiotic maturation of the starfish oocyte.
    Dev Biol. 1987 Nov;124(1):248-58 PMID: 15669148
  3. Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
    J Exp Zool. 1971 Jun;177(2):129-45 PMID: 5106340
  4. Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis.
    J Embryol Exp Morphol. 1978 Feb;43:157-66 PMID: 564937
  5. The induction of reversible and irreversible chromosome decondensation by protein synthesis inhibition during meiotic maturation of mouse oocytes.
    Dev Biol. 1983 Jun;97(2):291-301 PMID: 6682812
  6. Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
    J Cell Biol. 1984 Apr;98(4):1247-55 PMID: 6425302
  7. Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
    Mol Cell Biol. 1984 Aug;4(8):1631-4 PMID: 6436688
  8. Phosphorylation of ribosomal protein S6 on serine after microinjection of the Abelson murine leukemia virus tyrosine-specific protein kinase into Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):272-6 PMID: 3918307
  9. Expression of c-mos proto-oncogene transcripts in mouse tissues.
    Nature. 1985 Jun 6-12;315(6019):516-8 PMID: 4000280
  10. Nucleotide sequence and biochemical activities of the Moloney murine sarcoma virus strain HT-1 mos gene.
    J Virol. 1985 Oct;56(1):144-52 PMID: 2993645
  11. Regulation of a ribosomal protein S6 kinase activity by the Rous sarcoma virus transforming protein, serum, or phorbol ester.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7621-5 PMID: 3934663
  12. Changes in total RNA, polyadenylated RNA, and actin mRNA during meiotic maturation of mouse oocytes.
    Dev Biol. 1985 Apr;108(2):325-31 PMID: 2416609
  13. Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert.
    J Biol Chem. 1986 Jan 15;261(2):839-48 PMID: 3001084
  14. Increased phosphorylation of ribosomal protein S6 following microinjection of insulin receptor-kinase into Xenopus oocytes.
    Nature. 1986 Apr 3-9;320(6061):459-61 PMID: 3083266
  15. Widespread changes in the translation and adenylation of maternal messenger RNAs following fertilization of Spisula oocytes.
    Dev Biol. 1987 May;121(1):237-46 PMID: 3569660
  16. c-mos proto-oncogene RNA transcripts in mouse tissues: structural features, developmental regulation, and localization in specific cell types.
    Mol Cell Biol. 1987 May;7(5):1629-37 PMID: 3299051
  17. Expression of c-mos RNA in germ cells of male and female mice.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4509-13 PMID: 2955407
  18. Distinct developmental patterns of c-mos protooncogene expression in female and male mouse germ cells.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5301-5 PMID: 3474657
  19. Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
    Mol Cell Biol. 1987 Sep;7(9):3147-55 PMID: 3313008
  20. Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7896-900 PMID: 2825169
  21. Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
    Genes Dev. 1987 Dec;1(10):1201-11 PMID: 3123315
  22. Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation.
    Dev Biol. 1988 Apr;126(2):242-52 PMID: 3350209
  23. Chicken homolog of the mos proto-oncogene.
    Mol Cell Biol. 1988 Feb;8(2):923-9 PMID: 2832744
  24. Developmental regulation of ovarian-specific Mos expression.
    Oncogene. 1988 Mar;2(3):235-40 PMID: 3353114
  25. Regulation of MPF activity in vitro.
    Cell. 1988 Apr 22;53(2):185-95 PMID: 2834064
  26. The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
    Cell. 1988 Jul 29;54(3):423-31 PMID: 3293802
  27. Evidence for the involvement of the proto-oncogene c-mos in mammalian meiotic maturation and possibly very early embryogenesis.
    EMBO J. 1988 Mar;7(3):683-9 PMID: 2840283
  28. Post-transcriptional processing suggests that c-mos functions as a maternal message in mouse eggs.
    Oncogene. 1988 Aug;3(2):159-62 PMID: 2457860
  29. Identification of the protein product of the c-mos proto-oncogene in mouse testes.
    Oncogene. 1988 Aug;3(2):225-9 PMID: 2970613
  30. Changes in state of adenylation and time course of degradation of maternal mRNAs during oocyte maturation and early embryonic development in the mouse.
    Dev Biol. 1988 Oct;129(2):304-14 PMID: 2458285
  31. Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
    Nature. 1988 Oct 6;335(6190):519-25 PMID: 2971141
  32. Fertilization and cleavage of mouse oocytes exposed to the conditions of human oocyte retrieval for in vitro fertilization.
    Fertil Steril. 1989 Apr;51(4):675-81 PMID: 2494083
  33. Suppression of chromosome condensation during meiotic maturation induces parthenogenetic development of mouse oocytes.
    Development. 1988 Sep;104(1):97-103 PMID: 3253063
  34. Gene expression during oogenesis and oocyte development in mammals.
    Dev Biol (N Y 1985). 1985;1:453-524 PMID: 2481471
  35. The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
    Dev Biol. 1971 Jun;25(2):232-47 PMID: 5562852
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.7038
Published
1989-09-00
Pages
7038-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297988
Subset
IM
Grants
NCI NIH HHS · CA21082 · United States
NCI NIH HHS · CA28946 · United States
NICHD NIH HHS · HD21890 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.18.7038 PMC Full Text View journal details
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