Home LiteratureArticle Details
PMID: 6436688 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.

Molecular and cellular biology ·Vol. 4 ·No. 8 ·1984-08-00 ·Pages 1631-4

Spivack JG, Erikson RL, Maller JL

Abstract

Microinjection of purified pp60v-src into Xenopus oocytes caused the phosphorylation of ribosomal protein S6 on serine residues and also increased total protein phosphorylation, with almost a two-fold increase in the percentage of phosphotyrosine present. In addition, pp60v-src accelerated the time course of progesterone-induced oocyte maturation, suggesting that the biochemical pathway influenced by pp60v-src is related to that induced by progesterone.

MeSH Terms
Amino Acids/analysis Animals Electrophoresis, Polyacrylamide Gel Female Meiosis Microinjections Oncogene Protein pp60(v-src) Oocytes/cytology,drug effects,metabolism Phosphorylation Progesterone/pharmacology Protein Kinases/metabolism Ribosomal Protein S6 Ribosomal Proteins/isolation & purification,metabolism Ribosomes/metabolism Viral Proteins/metabolism Xenopus
Chemicals
Amino Acids Ribosomal Protein S6 Ribosomal Proteins Viral Proteins Progesterone Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Spivack J G
Erikson R L
Maller J L
References (22)
22 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cells.
    Mol Gen Genet. 1977 Apr 29;152(3):223-30 PMID: 876026
  3. Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
    Dev Biol. 1977 Jul 15;58(2):295-312 PMID: 885290
  4. Evolutionary microdivergence of chick and rat liver ribosomal proteins.
    J Biol Chem. 1977 Dec 25;252(24):9065-70 PMID: 925036
  5. Insulin-like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G1 phase of the cell cycle.
    FEBS Lett. 1979 Apr 1;100(1):185-90 PMID: 571354
  6. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  7. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  8. Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:907-17 PMID: 6253222
  9. Molecular events in cells transformed by Rous Sarcoma virus.
    J Cell Biol. 1980 NOV;87(2 Pt 1):319-25 PMID: 6253501
  10. Translational control in lysates of Drosophila melanogaster cells.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3353-7 PMID: 6267586
  11. Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4112-5 PMID: 6270659
  12. Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
    Eur J Biochem. 1981 Nov;120(2):351-5 PMID: 7318832
  13. The mechanism for increased protein synthesis during Xenopus oocyte maturation.
    Dev Biol. 1982 Jan;89(1):159-67 PMID: 7033020
  14. Preferential utilization of phosphorylated 40-S ribosomal subunits during initiation complex formation.
    Eur J Biochem. 1982 Apr;123(3):535-8 PMID: 7075598
  15. Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1781-5 PMID: 6804946
  16. Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2937-41 PMID: 7045876
  17. The effect of serum, EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis.
    Cell. 1982 Aug;30(1):235-42 PMID: 6751557
  18. Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):926-30 PMID: 6573662
  19. Platelet-derived growth factor stimulates the phosphorylation of ribosomal protein S6.
    FEBS Lett. 1983 May 30;156(1):130-4 PMID: 6852247
  20. Characterization of the Rous sarcoma virus transforming gene product.
    J Biol Chem. 1983 May 25;258(10):6344-51 PMID: 6304035
  21. Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6.
    J Biol Chem. 1983 Nov 25;258(22):14003-8 PMID: 6643464
  22. The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
    Dev Biol. 1984 Mar;102(1):79-89 PMID: 6365656
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.8.1631-1634.1984
Published
1984-08-00
Pages
1631-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368957
Subset
IM
Grants
NIADDK NIH HHS · AM-28353 · United States
NCI NIH HHS · CA-15823 · United States
NCI NIH HHS · CA-21117 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.8.1631-1634.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2