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PMID: 20930037 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Uncovering the human methyltransferasome.

Molecular & cellular proteomics : MCP ·Vol. 10 ·No. 1 ·2011-01-00 ·Pages M110.000976

Petrossian TC, Clarke SG

Abstract

We present a comprehensive analysis of the human methyltransferasome. Primary sequences, predicted secondary structures, and solved crystal structures of known methyltransferases were analyzed by hidden Markov models, Fisher-based statistical matrices, and fold recognition prediction-based threading algorithms to create a model, or profile, of each methyltransferase superfamily. These profiles were used to scan the human proteome database and detect novel methyltransferases. 208 proteins in the human genome are now identified as known or putative methyltransferases, including 38 proteins that were not annotated previously. To date, 30% of these proteins have been linked to disease states. Possible substrates of methylation for all of the SET domain and SPOUT methyltransferases as well as 100 of the 131 seven-β-strand methyltransferases were surmised from sequence similarity clusters based on alignments of the substrate-specific domains.

MeSH Terms
Computational Biology Humans Methyltransferases/chemistry,metabolism Protein Structure, Secondary Protein Structure, Tertiary Proteome/chemistry,metabolism Saccharomyces cerevisiae/enzymology Sequence Homology, Amino Acid Substrate Specificity
Chemicals
Proteome Methyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Petrossian Tanya C
Department of Chemistry and Biochemistry and Molecular Biology Institute, UCLA, Los Angeles, California 90095-1570, USA.
Clarke Steven G
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Article Info
Journal
Molecular & cellular proteomics : MCP
Abbr.
Mol Cell Proteomics
ISSN
1535-9484
DOI
10.1074/mcp.M110.000976
Published
2011-01-00
Epub
2010-00-07
Pages
M110.000976
Language
English
Region
United States
NLM ID
101125647
PMCID
PMC3013446
Subset
IM
Grants
NIGMS NIH HHS · R01 GM026020 · United States
NIGMS NIH HHS · R37 GM026020 · United States
NIGMS NIH HHS · GM026020 · United States
NIGMS NIH HHS · T32 GM008496 · United States
NIGMS NIH HHS · GM008496 · United States
External Links
PubMed source DOI: 10.1074/mcp.M110.000976 PMC Full Text View journal details
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