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PMID: 14704425 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme.

Science (New York, N.Y.) ·Vol. 303 ·No. 5654 ·2004-01-02 ·Pages 76-9

Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan CL

Abstract

The crystal structure of biotin synthase from Escherichia coli in complex with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4 angstrom resolution. This structure addresses how "AdoMet radical" or "radical SAM" enzymes use Fe4S4 clusters and S-adenosyl-L-methionine to generate organic radicals. Biotin synthase catalyzes the radical-mediated insertion of sulfur into dethiobiotin to form biotin. The structure places the substrates between the Fe4S4 cluster, essential for radical generation, and the Fe2S2 cluster, postulated to be the source of sulfur, with both clusters in unprecedented coordination environments.

MeSH Terms
Amino Acid Motifs Binding Sites Biotin/analogs & derivatives,chemistry,metabolism Catalysis Crystallization Crystallography, X-Ray Dimerization Escherichia coli/enzymology Escherichia coli Proteins/chemistry,metabolism Hydrogen/chemistry Hydrogen Bonding Iron/chemistry Ligands Models, Molecular Protein Binding Protein Conformation Protein Folding Protein Structure, Secondary Protein Structure, Tertiary S-Adenosylmethionine/chemistry,metabolism Sulfur/chemistry Sulfurtransferases/chemistry,metabolism
Chemicals
Escherichia coli Proteins Ligands Biotin Sulfur desthiobiotin S-Adenosylmethionine Hydrogen Iron Sulfurtransferases biotin synthase, E coli
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berkovitch Frederick
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Nicolet Yvain
Wan Jason T
Jarrett Joseph T
Drennan Catherine L
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1088493
Published
2004-01-02
Pages
76-9
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC1456065
Subset
IM
Grants
NIGMS NIH HHS · R01-GM59175 · United States
NINDS NIH HHS · NSLS X25 · United States
NIGMS NIH HHS · R01 GM059175 · United States
NIGMS NIH HHS · T32-GM07229 · United States
NIGMS NIH HHS · R01-GM65337 · United States
Databases
PDB
1R30
External Links
PubMed source DOI: 10.1126/science.1088493 PMC Full Text View journal details
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