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PMID: 18188184 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

CARM1 promotes adipocyte differentiation by coactivating PPARgamma.

EMBO reports ·Vol. 9 ·No. 2 ·2008-02-00 ·Pages 193-8

Yadav N, Cheng D, Richard S, Morel M, Iyer VR, Aldaz CM, Bedford MT

Abstract

The coactivator-associated arginine methyltransferase 1 (CARM1) is recruited to gene promoters by many transcription factors. To identify new pathways that use CARM1, we carried out a comprehensive transcriptome analysis of CARM1-knockout embryos. By using complementary DNA microarrays and serial analysis of gene expression, we identified various genes involved in lipid metabolism that were underrepresented in CARM1-knockout embryos, indicating an important role for this coactivator in adipose tissue biology. We also observed that the amount of brown fat in CARM1-knockout embryos is reduced. Furthermore, cells lacking CARM1 have a severely curtailed potential to differentiate into mature adipocytes. Reporter experiments and chromatin immunoprecipitation analysis show that CARM1 regulates these processes by acting as a coactivator for peroxisome proliferator-activated receptor gamma (PPARgamma). Together, these results show that CARM1 promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance.

MeSH Terms
3T3-L1 Cells Adipocytes/cytology,drug effects,enzymology Adipose Tissue, Brown/cytology,drug effects,embryology,enzymology Animals Cell Differentiation/drug effects Embryo, Mammalian/drug effects,enzymology Estrogens/pharmacology Gene Expression Profiling Mice PPAR gamma/metabolism Protein-Arginine N-Methyltransferases/metabolism RNA, Messenger/genetics,metabolism Transcription, Genetic/drug effects
Chemicals
Estrogens PPAR gamma RNA, Messenger Protein-Arginine N-Methyltransferases coactivator-associated arginine methyltransferase 1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yadav Neelu
Department of Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Science Park, Smithville, Texas 78957, USA.
Cheng Donghang
Richard Stephane
Morel Melanie
Iyer Vishwanath R
Aldaz C Marcelo
Bedford Mark T
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7401151
Published
2008-02-00
Epub
2008-00-11
Pages
193-8
Language
English
Region
England
NLM ID
100963049
PMCID
PMC2246418
Subset
IM
Grants
NIDDK NIH HHS · R01 DK062248 · United States
NIDDK NIH HHS · R56 DK062248 · United States
NCI NIH HHS · U01CA84243 · United States
NIDDK NIH HHS · DK62248 · United States
NCI NIH HHS · U01 CA084243 · United States
External Links
PubMed source DOI: 10.1038/sj.embor.7401151 PMC Full Text View journal details
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