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PMID: 20838597 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The baker's yeast diploid genome is remarkably stable in vegetative growth and meiosis.

PLoS genetics ·Vol. 6 ·No. 9 ·2010-09-09 ·Pages e1001109

Nishant KT, Wei W, Mancera E, Argueso JL, Schlattl A, Delhomme N, Ma X, Bustamante CD, Korbel JO, Gu Z, Steinmetz LM, Alani E

Abstract

Accurate estimates of mutation rates provide critical information to analyze genome evolution and organism fitness. We used whole-genome DNA sequencing, pulse-field gel electrophoresis, and comparative genome hybridization to determine mutation rates in diploid vegetative and meiotic mutation accumulation lines of Saccharomyces cerevisiae. The vegetative lines underwent only mitotic divisions while the meiotic lines underwent a meiotic cycle every ∼20 vegetative divisions. Similar base substitution rates were estimated for both lines. Given our experimental design, these measures indicated that the meiotic mutation rate is within the range of being equal to zero to being 55-fold higher than the vegetative rate. Mutations detected in vegetative lines were all heterozygous while those in meiotic lines were homozygous. A quantitative analysis of intra-tetrad mating events in the meiotic lines showed that inter-spore mating is primarily responsible for rapidly fixing mutations to homozygosity as well as for removing mutations. We did not observe 1-2 nt insertion/deletion (in-del) mutations in any of the sequenced lines and only one structural variant in a non-telomeric location was found. However, a large number of structural variations in subtelomeric sequences were seen in both vegetative and meiotic lines that did not affect viability. Our results indicate that the diploid yeast nuclear genome is remarkably stable during the vegetative and meiotic cell cycles and support the hypothesis that peripheral regions of chromosomes are more dynamic than gene-rich central sections where structural rearrangements could be deleterious. This work also provides an improved estimate for the mutational load carried by diploid organisms.

MeSH Terms
Amino Acid Substitution/genetics Chromosomes, Fungal/genetics Computer Simulation DNA, Fungal/genetics Diploidy Electrophoresis, Gel, Pulsed-Field Genome, Fungal/genetics Homozygote INDEL Mutation/genetics Karyotyping Meiosis/genetics Mutation/genetics Polymorphism, Genetic Reproduction/genetics Saccharomyces cerevisiae/cytology,genetics,growth & development Sequence Analysis, DNA Spores, Fungal/cytology,genetics
Chemicals
DNA, Fungal
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Nishant K T
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America.
Wei Wu
Mancera Eugenio
Argueso Juan Lucas
Schlattl Andreas
Delhomme Nicolas
Ma Xin
Bustamante Carlos D
Korbel Jan O
Gu Zhenglong
Steinmetz Lars M
Alani Eric
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1001109
Published
2010-09-09
Epub
2010-00-09
Pages
e1001109
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC2936533
Subset
IM
Grants
NIEHS NIH HHS · RC1 ES018091 · United States
NIGMS NIH HHS · R01 GM024110 · United States
NIEHS NIH HHS · 1RC1ES018091-01 · United States
NIGMS NIH HHS · R01 GM052319 · United States
NIGMS NIH HHS · GM52319 · United States
NIGMS NIH HHS · GM24110 · United States
NIGMS NIH HHS · R01 GM053085 · United States
NIGMS NIH HHS · GM53085 · United States
External Links
PubMed source DOI: 10.1371/journal.pgen.1001109 PMC Full Text View journal details
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