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PMID: 7672595 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA synthesis errors associated with double-strand-break repair.

Genetics ·Vol. 140 ·No. 3 ·1995-07-00 ·Pages 965-72

Strathern JN, Shafer BK, McGill CB

Abstract

Repair of a site-specific double-strand DNA break (DSB) resulted in increased reversion frequency for a nearby allele. Site-specific DSBs were introduced into the genome of Saccharomyces cerevisiae by the endonuclease encoded by the HO gene. Expression of the HO gene from a galactose-inducible promoter allowed efficient DNA cleavage at a single site in large populations of cells. To determine whether the DNA synthesis associated with repair of DSBs has a higher error rate than that associated with genome duplication, HO-induced DSBs were generated 0.3 kb from revertible alleles of trp1. The reversion rate of the trp1 alleles was approximately 100-fold higher among cells that had experienced an HO cut than among uninduced cells. The reverted allele was found predominantly on the chromosome that experienced the DNA cleavage.

Related Genes
HO trp1
MeSH Terms
Alleles Amino Acid Sequence Base Sequence Chromosomes, Fungal Crosses, Genetic Crossing Over, Genetic DNA Repair DNA, Fungal/biosynthesis DNA-Directed DNA Polymerase/metabolism Diploidy Frameshift Mutation Genes, Fungal Genotype Haploidy Molecular Sequence Data Mutagenesis Oligodeoxyribonucleotides Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism
Chemicals
DNA, Fungal Oligodeoxyribonucleotides DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Strathern J N
Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Maryland 21702-1201, USA.
Shafer B K
McGill C B
References (26)
26 references, click to expand
  1. Recombination initiated by double-strand breaks.
    Curr Genet. 1993;23(4):305-14 PMID: 8467528
  2. A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7831-5 PMID: 3020559
  3. DNA mismatch correction in a defined system.
    Science. 1989 Jul 14;245(4914):160-4 PMID: 2665076
  4. DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Apr;15(4):2173-9 PMID: 7891712
  5. Multi-stage proofreading in DNA replication.
    Q Rev Biophys. 1993 Aug;26(3):225-331 PMID: 8022969
  6. DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Feb;13(2):1051-8 PMID: 8423775
  7. Model for the participation of quasi-palindromic DNA sequences in frameshift mutation.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4128-32 PMID: 7051004
  8. Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences.
    Mol Cell Biol. 1988 Sep;8(9):3918-28 PMID: 3065627
  9. DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Feb;9(2):365-76 PMID: 2651896
  10. Rearrangements occurring adjacent to a single Ty1 yeast retrotransposon in the presence and absence of full-length Ty1 transcription.
    Genetics. 1992 Aug;131(4):833-50 PMID: 1325387
  11. GATC sequences, DNA nicks and the MutH function in Escherichia coli mismatch repair.
    EMBO J. 1987 Apr;6(4):1121-7 PMID: 2954815
  12. Recombination in the eukaryotic nucleus.
    Bioessays. 1988 Aug-Sep;9(2-3):61-4 PMID: 3066359
  13. Coconversion of flanking sequences with homothallic switching.
    Cell. 1989 May 5;57(3):459-67 PMID: 2541914
  14. A novel recombinator in yeast based on gene II protein from bacteriophage f1.
    Genetics. 1991 Jan;127(1):61-73 PMID: 2016047
  15. A DNA double chain break stimulates triparental recombination in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6225-9 PMID: 2668958
  16. Directionality and regulation of cassette substitution in yeast.
    Cold Spring Harb Symp Quant Biol. 1984;49:97-104 PMID: 6397325
  17. Mechanisms and biological effects of mismatch repair.
    Annu Rev Genet. 1991;25:229-53 PMID: 1812808
  18. Analysis of interchromosomal mitotic recombination.
    Curr Genet. 1990 Jul;18(1):29-39 PMID: 2173978
  19. Double-strand breaks can initiate meiotic recombination in S. cerevisiae.
    Cell. 1986 Aug 29;46(5):733-40 PMID: 3527443
  20. Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.
    EMBO J. 1990 Mar;9(3):663-73 PMID: 2178924
  21. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Mar;52(1):70-102 PMID: 3280967
  22. Molecular genetics of yeast mating type.
    Annu Rev Genet. 1982;16:439-500 PMID: 6760802
  23. Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.
    Methods Enzymol. 1991;194:319-29 PMID: 2005796
  24. Diploid yeast cells yield homozygous spontaneous mutations.
    Curr Genet. 1993 May-Jun;23(5-6):430-4 PMID: 8319299
  25. Different Rates of Spontaneous Mutation during Mitosis and Meiosis in Yeast.
    Genetics. 1962 Aug;47(8):1097-108 PMID: 17248123
  26. Double-strand breaks stimulate alternative mechanisms of recombination repair.
    J Mol Biol. 1989 Jun 5;207(3):527-41 PMID: 2668534
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/140.3.965
Published
1995-07-00
Pages
965-72
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206680
Subset
IM
Grants
NCI NIH HHS · N01-CO-46000 · United States
External Links
PubMed source DOI: 10.1093/genetics/140.3.965 PMC Full Text View journal details
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