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PMID: 3020559 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7831-5

Nickoloff JA, Chen EY, Heffron F

Abstract

HO nuclease is a site-specific double-strand endonuclease present in haploid Saccharomyces cerevisiae undergoing mating type interconversion. HO nuclease initiates mating type interconversion by making a double-strand break within the MAT locus. To define the recognition site for the enzyme in vitro, we have constructed a number of point mutations and deletions within or adjacent to the HO recognition site. Digestion of these substrates with HO in vitro reveals that the minimal recognition site is 18 base pairs long, although several shorter substrates and substrates containing point mutations are cleaved at low levels in vitro. A 24-base-pair HO recognition site stimulates homologous recombination when present in a region unrelated to MAT. Recombinants arise from both gene conversion and crossover events. The identification of the HO recognition site provides a way of introducing a defined initiation site for recombination.

MeSH Terms
Alleles Base Composition Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Fungal/analysis Histidine/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal Histidine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nickoloff J A
Chen E Y
Heffron F
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7831
Published
1986-10-00
Pages
7831-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386816
Subset
IM
Grants
NIGMS NIH HHS · R01 GM33808-1 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.20.7831 PMC Full Text View journal details
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