Home LiteratureArticle Details
PMID: 8467528 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recombination initiated by double-strand breaks.

Current genetics ·Vol. 23 ·No. 4 ·1993-00-00 ·Pages 305-14

McGill CB, Shafer BK, Derr LK, Strathern JN

Abstract

The HO endonuclease was used to introduce a site-specific double-strand break (DSB) in an interval designed to monitor mitotic recombination. The interval included the trp1 and his3 genes inserted into chromosome III of S. cerevisiae between the CRY1 and MAT loci. Mitotic recombination was monitored in a diploid carrying heteroalleles of trp1 and his3. The normal recognition sites for the HO endonuclease were mutated at the MAT alleles and a synthetic recognition site for HO endonuclease was placed between trp1 and his3 on one of the chromosomes. HO-induced cleavage resulted in efficient recombination in this interval. Most of the data can be explained by double-strand gap repair in which the cut chromosome acts as the recipient. However, analysis of some of the recombinants indicates that regions of heteroduplex were generated flanking the site of the cut, and that some recombinants were the result of the cut chromosome acting as the genetic donor.

Related Genes
CRY1 MAT his3 trp1
MeSH Terms
Base Sequence DNA DNA Damage Deoxyribonucleases, Type II Site-Specific/metabolism Molecular Sequence Data Nucleic Acid Heteroduplexes Oligonucleotides Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Nucleic Acid Heteroduplexes Oligonucleotides Saccharomyces cerevisiae Proteins DNA HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McGill C B
Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research, MD.
Shafer B K
Derr L K
Strathern J N
References (32)
32 references, click to expand
  1. A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7831-5 PMID: 3020559
  2. The repair of double-strand breaks in DNA; a model involving recombination.
    J Theor Biol. 1976 Jun;59(1):97-106 PMID: 940351
  3. An alpha mating-type allele insensitive to the mutagenic action of the homothallic gene system in Saccharomyces diastaticus.
    Mol Gen Genet. 1973 Oct 16;126(1):19-28 PMID: 4777791
  4. Gene conversion adjacent to regions of double-strand break repair.
    Mol Cell Biol. 1988 Dec;8(12):5292-8 PMID: 3072478
  5. Recombination in the eukaryotic nucleus.
    Bioessays. 1988 Aug-Sep;9(2-3):61-4 PMID: 3066359
  6. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus.
    Cell. 1982 Nov;31(1):183-92 PMID: 6297747
  7. Coconversion of flanking sequences with homothallic switching.
    Cell. 1989 May 5;57(3):459-67 PMID: 2541914
  8. A novel recombinator in yeast based on gene II protein from bacteriophage f1.
    Genetics. 1991 Jan;127(1):61-73 PMID: 2016047
  9. A DNA double chain break stimulates triparental recombination in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6225-9 PMID: 2668958
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Intra-chromosomal gene conversion induced by a DNA double-strand break in Saccharomyces cerevisiae.
    J Mol Biol. 1988 May 20;201(2):247-60 PMID: 3047399
  12. Analysis of the HO-cleaved MAT DNA intermediate generated during the mating type switch in the yeast Saccharomyces cerevisiae.
    Mol Gen Genet. 1989 Dec;220(1):33-42 PMID: 2558285
  13. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  14. Mutations preventing transpositions of yeast mating type alleles.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2824-8 PMID: 6248869
  15. Physical monitoring of mating type switching in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jun;8(6):2342-9 PMID: 2841579
  16. Directionality and regulation of cassette substitution in yeast.
    Cold Spring Harb Symp Quant Biol. 1984;49:97-104 PMID: 6397325
  17. Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.
    Cell. 1991 Mar 22;64(6):1155-61 PMID: 2004421
  18. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  19. Analysis of interchromosomal mitotic recombination.
    Curr Genet. 1990 Jul;18(1):29-39 PMID: 2173978
  20. Double-strand breaks can initiate meiotic recombination in S. cerevisiae.
    Cell. 1986 Aug 29;46(5):733-40 PMID: 3527443
  21. Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.
    EMBO J. 1990 Mar;9(3):663-73 PMID: 2178924
  22. Heteroduplex formation and mismatch repair of the "stuck" mutation during mating-type switching in Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Oct;11(10):5372-80 PMID: 1922052
  23. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7585-9 PMID: 1881899
  24. DNA double-chain breaks in recombination of phage lambda and of yeast.
    Annu Rev Genet. 1988;22:169-97 PMID: 2977087
  25. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Mar;52(1):70-102 PMID: 3280967
  26. Molecular genetics of yeast mating type.
    Annu Rev Genet. 1982;16:439-500 PMID: 6760802
  27. Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.
    Methods Enzymol. 1991;194:319-29 PMID: 2005796
  28. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  29. Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
    Gene. 1989 Apr 15;77(1):51-9 PMID: 2744487
  30. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  31. Evidence for a physical interaction between the transposed and the substituted sequences during mating type gene transposition in yeast.
    Cell. 1980 Nov;22(1 Pt 1):291-8 PMID: 6253082
  32. Double-strand breaks stimulate alternative mechanisms of recombination repair.
    J Mol Biol. 1989 Jun 5;207(3):527-41 PMID: 2668534
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00310891
Published
1993-00-00
Pages
305-14
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
External Links
PubMed source DOI: 10.1007/BF00310891 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2