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PMID: 1996317 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inducible proteins binding to the murine thymidine kinase promoter in late G1/S phase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 4 ·1991-02-15 ·Pages 1157-61

Dou QP, Fridovich-Keil JL, Pardee AB

Abstract

By performing DNase I footprint and band-shift analyses of a 170-base-pair region of the murine thymidine kinase promoter, we identified an inducible DNA binding activity that we named Yi. Yi binding activity was not detected in G0 and G1 extracts, but it was observed as cells crossed the G1/S boundary. Yi proteins bind specifically to a consensus sequence (CCCNCNNNCT) found at three distinct sites in this promoter region. We also observed a murine Sp1 binding activity that was constitutive throughout the cell cycle. We propose that the G1/S-specific Yi binding is important for murine thymidine kinase gene regulation and perhaps also for initiation of DNA synthesis.

MeSH Terms
Animals Base Sequence Cell Line DNA/genetics,isolation & purification DNA-Binding Proteins/biosynthesis,metabolism G1 Phase Mice Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes Promoter Regions, Genetic S Phase Thymidine Kinase/genetics
Chemicals
DNA-Binding Proteins Oligonucleotide Probes DNA Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dou Q P
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA.
Fridovich-Keil J L
Pardee A B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.4.1157
Published
1991-02-15
Pages
1157-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50976
Subset
IM
Grants
NCI NIH HHS · CA22427 · United States
NIGMS NIH HHS · GM24571 · United States
Databases
GENBANK
M22581, S74646, S74648, S74650, S74653, S74655, S74658, S74661, S74663, X12824
External Links
PubMed source DOI: 10.1073/pnas.88.4.1157 PMC Full Text View journal details
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