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PMID: 2355925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter.

Molecular and cellular biology ·Vol. 10 ·No. 7 ·1990-07-00 ·Pages 3834-7

Roehl HH, Conrad SE

Abstract

We have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G1-S-phase regulation to the linked neo gene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G1- and S-phase cells and in the various deletion mutants tested.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Gene Expression Regulation, Enzymologic Genes Humans Interphase Molecular Sequence Data Mutation Oligonucleotide Probes Promoter Regions, Genetic Rats Thymidine Kinase/genetics Transfection
Chemicals
Oligonucleotide Probes Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roehl H H
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Conrad S E
References (19)
19 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.7.3834-3837.1990
Published
1990-07-00
Pages
3834-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360850
Subset
IM
Grants
NCI NIH HHS · CA37144 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.7.3834-3837.1990 PMC Full Text View journal details
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