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PMID: 1986221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 213-7

Karlsson R, Aspenström P, Byström AS

Abstract

Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.

Related Genes

ACT1

MeSH Terms

Actins/chemistry,genetics Animals Blotting, Southern Chickens/genetics Cloning, Molecular DNA Mutational Analysis Genetic Complementation Test Isoelectric Point Saccharomyces cerevisiae/cytology,genetics

Chemicals

Actins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Karlsson R

Department of Zoological Cell Biology, University of Stockholm, Sweden.

Aspenström P

Byström A S

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27 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.11.1.213-217.1991

Published

1991-01-00

Pages

213-7

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC359611

Subset

External Links

PubMed source DOI: 10.1128/mcb.11.1.213-217.1991 PMC Full Text View journal details

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A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene.

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