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PMID: 3322809 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The yeast MYO1 gene encoding a myosin-like protein required for cell division.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3499-505

Watts FZ, Shiels G, Orr E

Abstract

A yeast gene MYO1 that contains regions of substantial sequence homology with the nematode muscle myosin gene (unc54) has been isolated and sequenced. Although the disruption of MYO1 is not lethal, it leads to aberrant nuclear migration and cytokinesis. The 200-kd myosin heavy chain-like protein, the product of MYO1, cross-reacts with anti-nematode myosin heavy chain IgG and is present in wild-type strains but not in strains carrying the disrupted gene. Instead, a truncated polypeptide with a molecular mass of 120 kd can be detected in some myo1 mutants.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Division Fungal Proteins/genetics Genes Genes, Fungal Genotype Molecular Sequence Data Myosins/genetics Nematoda Rabbits Saccharomyces cerevisiae/cytology,genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Fungal Proteins Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Watts F Z
Department of Genetics, University of Leicester, UK.
Shiels G
Orr E
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25 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3499-505
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553809
Subset
IM
Databases
GENBANK
X06187
External Links
PubMed source PMC Full Text View journal details
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