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PMID: 368805 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of tubulin from the yeast Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4962-6

Baum P, Thorner J, Honig L

Abstract

A tubulin-like protein was identified in the lower eukaryote Saccharomyces cerevisiae. The following criteria were used: (i) copolymerization of the 35S-labeled yeast protein with porcine brain tubulin; (ii) immunoprecipitation of the 35S-labeled yeast protein with antiflagellar tubulin antibody; (iii) the presence of the yeast protein as a constituent of isolated yeast nuclei; and (iv) splitting of the yeast protein in a gel electrophoretic system containing sodium dodecyl sulfate that resolved the alpha- and beta-tubulin chains from other sources. This protein did not appear to have significant affinity for the plant alkaloid, Colcemid.

MeSH Terms
Cell Nucleus/analysis Chromatography, Gel Demecolcine/metabolism Glycoproteins/analysis Radioimmunoassay Saccharomyces cerevisiae/analysis Tubulin/analysis
Chemicals
Glycoproteins Tubulin Demecolcine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baum P
Thorner J
Honig L
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4962
Published
1978-10-00
Pages
4962-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336242
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.4962 PMC Full Text View journal details
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