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PMID: 2994062 Published · ppublish English Comparative Study Journal Article

Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6133-7

Nakajima-Iijima S, Hamada H, Reddy P, Kakunaga T

Abstract

A recombinant phage that carries the cytoplasmic beta-actin gene was isolated from a human DNA library. The nucleotide sequence of this gene was determined. The amino acid sequence deduced from the nucleotide sequence matches perfectly that of beta-actin from human fibroblasts. The gene contains five introns. A large intron was found in the 5' untranslated region six nucleotides upstream from the ATG initiation codon. Four introns were found within the coding region at codons specifying amino acids 41/42, 121/122, 267, and 327/328. In contrast to the human cardiac muscle actin gene, the aorta-type smooth muscle actin gene, and the stomach-type smooth muscle actin gene, the beta-actin gene lacks the codon for cysteine between the ATG initiation codon and the codon for the NH2-terminal amino acid of the mature protein. Hybridization of genomic DNA with DNA fragments derived from intron I in the 5' untranslated region and from intron III strongly suggests the presence of a single beta-actin gene in the human genome. The DNA sequences of the coding region, of the 3' untranslated region, and of the sequence block between the "CCAAT" box and "TATA" box in the 5' flanking DNA of the human beta-actin gene are highly homologous to the corresponding sequences of the rat and chicken beta-actin genes. Unexpectedly, the sequence of intron III of the human beta-actin gene shows considerable homology to that of the rat beta-actin gene.

MeSH Terms
Actins/genetics Animals Base Sequence Cytoplasm DNA Restriction Enzymes Genes Humans Plasmids Promoter Regions, Genetic Protein Biosynthesis RNA, Messenger/genetics
Chemicals
Actins RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nakajima-Iijima S
Hamada H
Reddy P
Kakunaga T
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6133
Published
1985-09-00
Pages
6133-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391006
Subset
IM
Databases
GENBANK
M10277
External Links
PubMed source DOI: 10.1073/pnas.82.18.6133 PMC Full Text View journal details
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