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PMID: 7111028 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence of a sea urchin actin gene.

Nucleic acids research ·Vol. 10 ·No. 13 ·1982-07-10 ·Pages 4081-92

Cooper AD, Crain WR

Abstract

We have determined the complete nucleotide sequence of a sea urchin actin gene, including the entire protein-coding sequence, introns and approximately 500 and 700 nucleotides adjacent to protein-coding-sequence on the 5' and 3' sides, respectively. This gene is split between codons 121 and 122 and within codon 204 by two introns which are 233 and 181 nucleotides in length, respectively. Comparison of the sequence of the two introns indicates a region of distant relatedness which covers about 25% of their lengths, suggesting that these sequences might have derived from a common ancestral sequence. The encoded amino acid sequence, which matches plasmic-like than muscle-like when compared to vertebrate actins. Analysis of the coding-flanking regions indicates the presence of sequences similar to those thought to be important for initiation of transcription and polyadenylation of mRNA. The location of these sequences and the size of an actin mRNA, transcribed from this or a very closely related gene, suggests that initiation occurs 347 nucleotides 5' of coding and polyadenylation approximately 515 nucleotides 3' of coding.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Codon/genetics Genes Molecular Sequence Data RNA, Messenger/genetics Sea Urchins/genetics Species Specificity Transcription, Genetic
Chemicals
Actins Codon RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cooper A D
Crain W R
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.13.4081
Published
1982-07-10
Pages
4081-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320780
Subset
IM
Databases
GENBANK
J01202
External Links
PubMed source DOI: 10.1093/nar/10.13.4081 PMC Full Text View journal details
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