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PMID: 19101707 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

SV40 DNA replication: from the A gene to a nanomachine.

Virology ·Vol. 384 ·No. 2 ·2009-02-20 ·Pages 352-9

Fanning E, Zhao K

Abstract

Duplication of the simian virus 40 (SV40) genome is the best understood eukaryotic DNA replication process to date. Like most prokaryotic genomes, the SV40 genome is a circular duplex DNA organized in a single replicon. This small viral genome, its association with host histones in nucleosomes, and its dependence on the host cell milieu for replication factors and precursors led to its adoption as a simple and powerful model. The steps in replication, the viral initiator, the host proteins, and their mechanisms of action were initially defined using a cell-free SV40 replication reaction. Although our understanding of the vastly more complex host replication fork is advancing, no eukaryotic replisome has yet been reconstituted and the SV40 paradigm remains a point of reference. This article reviews some of the milestones in the development of this paradigm and speculates on its potential utility to address unsolved questions in eukaryotic genome maintenance.

MeSH Terms
Antigens, Polyomavirus Transforming/metabolism DNA Replication DNA, Viral/genetics Genome, Viral Simian virus 40/genetics,metabolism,physiology Virus Replication
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fanning Ellen
Department of Biological Sciences, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235-1634, USA. ellen.fanning@vanderbilt.edu
Zhao Kun
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
1096-0341
DOI
10.1016/j.virol.2008.11.038
Email
ellen.fanning@vanderbilt.edu
Published
2009-02-20
Epub
2008-00-20
Pages
352-9
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC2718763
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052948-14 · United States
NCI NIH HHS · P30 CA68485 · United States
NIGMS NIH HHS · GM52948 · United States
NCI NIH HHS · P30 CA068485 · United States
NIGMS NIH HHS · R01 GM052948-13 · United States
NIGMS NIH HHS · R01 GM052948 · United States
External Links
PubMed source DOI: 10.1016/j.virol.2008.11.038 PMC Full Text
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