Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2913-7

Danna K, Nathans D

Abstract

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.

MeSH Terms

Acrylamides Animals Autoradiography Biodegradation, Environmental Carbon Isotopes Cell Line Centrifugation, Density Gradient DNA, Viral/analysis,metabolism Deoxyribonucleases Detergents Electrophoresis Haemophilus influenzae/enzymology Haplorhini Kidney Microscopy, Electron Molecular Weight Phosphorus Isotopes Simian virus 40/metabolism Structure-Activity Relationship Sulfates Tritium

Chemicals

Acrylamides Carbon Isotopes DNA, Viral Detergents Phosphorus Isotopes Sulfates Tritium Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Danna K

Nathans D

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.2913

Published

1971-12-00

Pages

2913-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389558

Subset

Corrections

CommentIn

PMID: 15840710

CommentIn

PMID: 15840723

External Links

PubMed source DOI: 10.1073/pnas.68.12.2913 PMC Full Text View journal details

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Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

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