Home LiteratureArticle Details
PMID: 1909999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation, characterization, and molecular cloning of a specific Mycobacterium tuberculosis antigen gene: identification of a species-specific sequence.

Infection and immunity ·Vol. 59 ·No. 10 ·1991-10-00 ·Pages 3411-7

Parra CA, Londoño LP, Del Portillo P, Patarroyo ME

Abstract

A rabbit polyclonal antiserum exhibiting a specific recognition pattern for Mycobacterium tuberculosis proteins was used to screen an M. tuberculosis genomic library constructed in the expression vector lambda gt11. One clone, denominated C1:10, expressed M. tuberculosis-specific determinants as part of a large fusion protein with beta-galactosidase. The gene for this protein has been sequenced, and it encodes a protein of 134 amino acids (13.8 kDa) which did not display significant homology with any of the previously reported proteins in the data bases. Hybridization studies with restriction fragments of the cloned sequence revealed that it was not present in the genomes of related mycobacteria, namely, M. bovis, M. bovis BCG, M. flavescens, M. fortuitum, M. phlei, and M. vaccae. These findings suggest that we have detected a gene, or a fragment therefrom, unique for M. tuberculosis whose nucleotide and amino acid sequences could be useful tools in the design of an improved vaccine or a diagnostic method of greater accuracy for tuberculosis.

MeSH Terms
Amino Acid Sequence Animals Antigens, Bacterial/genetics Base Sequence Cloning, Molecular Codon Gene Library Genes, Bacterial Immune Sera/immunology Molecular Sequence Data Mycobacterium tuberculosis/genetics Nucleic Acid Hybridization Rabbits Species Specificity
Chemicals
Antigens, Bacterial Codon Immune Sera
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parra C A
Instituto de Inmunología, Hospital San Juan de Dios, Universidad Nacional de Colombia, Bogotá.
Londoño L P
Del Portillo P
Patarroyo M E
References (39)
39 references, click to expand
  1. The antigens of Mycobacterium bovis, strain BCG, studied by crossed immunoelectrophoresis: a reference system.
    Scand J Immunol. 1980;12(3):249-63 PMID: 7008186
  2. Cascades of Sigma factors.
    Cell. 1981 Sep;25(3):582-4 PMID: 6793235
  3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  4. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  5. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  8. Comparison of antigens in sonic and pressure cell extracts of Mycobacterium tuberculosis.
    Infect Immun. 1976 Feb;13(2):425-37 PMID: 816743
  9. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  10. Identification of B- and T-cell epitopes within the MTP40 protein of Mycobacterium tuberculosis and their correlation with the disease course.
    Infect Immun. 1991 Jul;59(7):2265-73 PMID: 1711013
  11. Amplification of a species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis.
    J Clin Microbiol. 1991 Oct;29(10):2163-8 PMID: 1939567
  12. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  13. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen.
    J Bacteriol. 1988 Sep;170(9):3847-54 PMID: 2842287
  14. The 65-kilodalton antigen of Mycobacterium tuberculosis.
    J Bacteriol. 1987 Mar;169(3):1080-8 PMID: 3029018
  15. A new and fast method for preparing high quality lambda DNA suitable for sequencing.
    Nucleic Acids Res. 1988 Apr 11;16(7):2873-84 PMID: 2966928
  16. A gene from Mycobacterium tuberculosis which is homologous to the DnaJ heat shock protein of E. coli.
    Nucleic Acids Res. 1988 Feb 25;16(4):1636 PMID: 3126487
  17. Stress proteins are immune targets in leprosy and tuberculosis.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267-70 PMID: 3132709
  18. Immunogenic synthetic peptides against mycobacteria of potential immunodiagnostic and immunoprophylactic value.
    Lepr Rev. 1986 Dec;57 Suppl 2:163-8 PMID: 3106735
  19. Antigenic proteins of Mycobacterium leprae. Complete sequence of the gene for the 18-kDa protein.
    J Immunol. 1988 Jan 15;140(2):597-601 PMID: 2447183
  20. Results of a World Health Organization-sponsored workshop on monoclonal antibodies to Mycobacterium leprae.
    Infect Immun. 1985 May;48(2):603-5 PMID: 2580798
  21. Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2583-7 PMID: 2581251
  22. Genetic relationships among Mycobacterium leprae, Mycobacterium tuberculosis, and candidate leprosy vaccine strains determined by DNA hybridization: identification of an M. leprae-specific repetitive sequence.
    Infect Immun. 1989 May;57(5):1535-41 PMID: 2565292
  23. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis.
    Infect Immun. 1989 Oct;57(10):3123-30 PMID: 2506131
  24. Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock protein 70 of eucaryotes.
    Infect Immun. 1989 Jan;57(1):204-12 PMID: 2491836
  25. Approaches to the development of new antituberculosis drugs.
    Rev Infect Dis. 1989 Mar-Apr;11 Suppl 2:S467-70 PMID: 2496458
  26. Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG.
    Infect Immun. 1989 Jan;57(1):283-8 PMID: 2642468
  27. A species-specific repetitive sequence in Mycobacterium leprae DNA.
    J Infect Dis. 1989 Jan;159(1):7-15 PMID: 2642523
  28. A rapid procedure for colony screening using nylon filters.
    Nucleic Acids Res. 1989 Jan 11;17(1):452 PMID: 2911481
  29. The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein.
    Infect Immun. 1988 Feb;56(2):446-51 PMID: 2892795
  30. Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12.
    Infect Immun. 1987 Jun;55(6):1466-75 PMID: 3553003
  31. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.
    Infect Immun. 1986 Apr;52(1):293-302 PMID: 3514457
  32. Results of a World Health Organization-sponsored workshop to characterize antigens recognized by mycobacterium-specific monoclonal antibodies.
    Infect Immun. 1986 Feb;51(2):718-20 PMID: 3943911
  33. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
    J Bacteriol. 1985 Mar;161(3):1093-102 PMID: 3882664
  34. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  35. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  36. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  37. Graphic methods to determine the function of nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521-38 PMID: 6364040
  38. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  39. Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6999-7003 PMID: 6960359
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.10.3411-3417.1991
Published
1991-10-00
Pages
3411-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258900
Subset
IM
Databases
GENBANK
M57952, S54082, S61892, S70201, S70204, X06392, X14442, X16435, X53033, X57105, X59240
External Links
PubMed source DOI: 10.1128/iai.59.10.3411-3417.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2