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PMID: 3553003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12.

Infection and immunity ·Vol. 55 ·No. 6 ·1987-06-00 ·Pages 1466-75

Thole JE, Keulen WJ, De Bruyn J, Kolk AH, Groothuis DG, Berwald LG, Tiesjema RH, van Embden JD

Abstract

We report the DNA sequence of a previously cloned Mycobacterium bovis BCG gene encoding an immunogenic 64-kilodalton protein. This protein, MbaA, was purified from overproducing Escherichia coli K-12 cells, and the presence of antibodies to MbaA in human sera was determined by an enzyme-linked immunosorbent assay. In about 80% of serum samples from tuberculosis patients and in about 60% of samples from BCG-vaccinated individuals, significant levels of anti-MbaA antibodies were found. Surprisingly, in about 30% of the control serum samples obtained from children, anti-MbaA antibodies were also observed. Guinea pigs sensitized with M. bovis BCG or MbaA showed a delayed-type hypersensitivity reaction after challenge with purified MbaA, supporting the previously observed strong reactivity of human T-cell clones with this, for mycobacteria, common antigen.

MeSH Terms
Animals Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antibody Formation Bacterial Proteins/genetics,immunology,isolation & purification Base Sequence Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Escherichia coli/genetics Guinea Pigs Humans Mycobacterium bovis/genetics Tuberculosis, Pulmonary/immunology Vaccination
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Bacterial Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Thole J E
Keulen W J
De Bruyn J
Kolk A H
Groothuis D G
Berwald L G
Tiesjema R H
van Embden J D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.6.1466-1475.1987
Published
1987-06-00
Pages
1466-75
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260538
Subset
IM
Databases
GENBANK
M17705
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/iai.55.6.1466-1475.1987 PMC Full Text View journal details
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