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PMID: 18989454 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Silent but not static: accelerated base-pair substitution in silenced chromatin of budding yeasts.

PLoS genetics ·Vol. 4 ·No. 11 ·2008-11-00 ·Pages e1000247

Teytelman L, Eisen MB, Rine J

Abstract

Subtelomeric DNA in budding yeasts, like metazoan heterochromatin, is gene poor, repetitive, transiently silenced, and highly dynamic. The rapid evolution of subtelomeric regions is commonly thought to arise from transposon activity and increased recombination between repetitive elements. However, we found evidence of an additional factor in this diversification. We observed a surprising level of nucleotide divergence in transcriptionally silenced regions in inter-species comparisons of Saccharomyces yeasts. Likewise, intra-species analysis of polymorphisms also revealed increased SNP frequencies in both intergenic and synonymous coding positions of silenced DNA. This analysis suggested that silenced DNA in Saccharomyces cerevisiae and closely related species had increased single base-pair substitution that was likely due to the effects of the silencing machinery on DNA replication or repair.

MeSH Terms
Base Sequence Chromatin/genetics Conserved Sequence DNA, Fungal/genetics DNA, Intergenic/genetics Fungal Proteins/genetics Gene Silencing Molecular Sequence Data Point Mutation Polymorphism, Single Nucleotide Saccharomyces/genetics Telomere/genetics Transcription, Genetic
Chemicals
Chromatin DNA, Fungal DNA, Intergenic Fungal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Teytelman Leonid
Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, California, United States of America.
Eisen Michael B
Rine Jasper
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1000247
Published
2008-11-00
Epub
2008-00-07
Pages
e1000247
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC2570616
Subset
IM
Grants
NIGMS NIH HHS · R37 GM031105 · United States
NHGRI NIH HHS · R01 HG002779 · United States
NIGMS NIH HHS · R01 GM031105 · United States
NHGRI NIH HHS · R01-HG002779 · United States
NIGMS NIH HHS · GM31105 · United States
External Links
PubMed source DOI: 10.1371/journal.pgen.1000247 PMC Full Text View journal details
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