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PMID: 1898926 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.

Journal of bacteriology ·Vol. 173 ·No. 1 ·1991-01-00 ·Pages 46-54

Pang AS, Nathoo S, Wong SL

Abstract

Two novel Bacillus subtilis genes that regulate the production of several extracellular enzymes were clones and characterized. These two genes are organized as part of an operon. When cloned in a multicopy plasmid, the first gene (tenA, transcription enhancement) stimulates alkaline protease production at the transcriptional level. The second gene (tenI) exerts an opposite effect to reduce alkaline protease production. The production of neutral protease, levansucrase, and alkaline protease can be stimulated up to 11- to 55-fold. Thus, tenA is a new member of the deg (regulatory genes for degradative enzymes) family in B. subtilis. A functional degS product is required to observe the stimulatory effect from tenA. Between the promoter and the ribosome-binding site of tenA, there exists a terminatorlike structure. Deletion of this structure doubles the expression of tenA. Neither tenA nor tenI is essential for cell growth and the production of extracellular enzymes. However, inactivation of these genes causes a delay in sporulation. This operon is located close to tre on the genetic linkage map. The overall organization of this operon and its relationship with other known regulatory factors in the deg family are discussed.

Related Genes
tenA tenI
MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics,physiology Base Sequence Cloning, Molecular Endopeptidases/genetics,metabolism Genes, Bacterial Kinetics Molecular Sequence Data Operon Plasmids Promoter Regions, Genetic Protein Biosynthesis Restriction Mapping Spores, Bacterial/physiology Terminator Regions, Genetic Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
beta-Galactosidase Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pang A S
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Nathoo S
Wong S L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.1.46-54.1991
Published
1991-01-00
Pages
46-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207154
Subset
IM
Databases
GENBANK
M37776
External Links
PubMed source DOI: 10.1128/jb.173.1.46-54.1991 PMC Full Text View journal details
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