Home LiteratureArticle Details
PMID: 3007431 Published · ppublish English Journal Article

Identification of the pleiotropic sacQ gene of Bacillus subtilis.

Journal of bacteriology ·Vol. 166 ·No. 1 ·1986-04-00 ·Pages 113-9

Yang M, Ferrari E, Chen E, Henner DJ

Abstract

The sacQ gene of Bacillus subtilis, a pleiotropic gene affecting the expression of a number of secreted gene products, has been identified as a small 46-amino-acid polypeptide. The increased expression of this polypeptide in strains carrying the sacQ36 allele, or in strains carrying the sacQ gene on a high copy plasmid, appears to be responsible for the phenotype of higher levels of proteases seen in these strains. A deletion of the sacQ gene had no apparent phenotype, indicating that it is not an essential gene.

MeSH Terms
Alleles Bacillus subtilis/genetics Bacterial Proteins/analysis Base Sequence Genes, Bacterial Genes, Regulator Mutation Peptide Hydrolases/biosynthesis Plasmids RNA, Messenger/analysis Transcription, Genetic Transformation, Bacterial
Chemicals
Bacterial Proteins RNA, Messenger Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang M
Ferrari E
Chen E
Henner D J
References (36)
36 references, click to expand
  1. Cloning and expression in Escherichia coli of the regulatory sacU gene from Bacillus subtilis.
    J Bacteriol. 1985 Mar;161(3):1182-7 PMID: 3918985
  2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  3. Structure-independent nucleotide sequence analysis.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2232-5 PMID: 287063
  4. Construction and properties of an integrable plasmid for Bacillus subtilis.
    J Bacteriol. 1983 Jun;154(3):1513-5 PMID: 6304019
  5. Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg.
    J Bacteriol. 1975 Feb;121(2):688-94 PMID: 803484
  6. The 64-kilodalton membrane protein of Bacillus subtilis is also present as a multiprotein complex on membrane-free ribosomes.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7772-6 PMID: 6440144
  7. Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168.
    Biochimie. 1974;56(11-12):1481-9 PMID: 4219582
  8. Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes.
    J Biol Chem. 1982 Jan 25;257(2):1053-62 PMID: 6274853
  9. Extracellular enzyme synthesis in the genus Bacillus.
    Bacteriol Rev. 1977 Sep;41(3):711-53 PMID: 334155
  10. Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
    J Bacteriol. 1967 Jun;93(6):1925-37 PMID: 4960898
  11. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  12. Bacillus subtilis requires a "stringent" Shine-Dalgarno region for gene expression.
    DNA. 1984;3(1):17-21 PMID: 6199171
  13. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  14. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  15. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  16. A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins.
    Cell. 1983 Mar;32(3):789-97 PMID: 6339072
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.
    Cell. 1983 Nov;35(1):275-83 PMID: 6414720
  20. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.
    J Biol Chem. 1981 Nov 10;256(21):11283-91 PMID: 6793593
  21. Secretory S complex of Bacillus subtilis forms a large, organized structure when released from ribosomes.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4031-5 PMID: 3923486
  22. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
    J Bacteriol. 1984 Oct;160(1):15-21 PMID: 6090407
  23. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  24. Construction of plasmids carrying the cI gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
  25. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis.
    Mol Gen Genet. 1982;186(3):339-46 PMID: 6181373
  26. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  27. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.
    Mol Gen Genet. 1972;118(2):135-60 PMID: 4628133
  28. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  29. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome.
    J Bacteriol. 1975 Mar;121(3):823-34 PMID: 803963
  30. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  31. Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli.
    J Bacteriol. 1982 May;150(2):686-91 PMID: 6279567
  32. Thermosensitive, extracellular neutral proteases in Bacillus subtilis: isolation, characterization, and genetics.
    J Bacteriol. 1979 Aug;139(2):583-90 PMID: 37241
  33. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168.
    Appl Environ Microbiol. 1977 Apr;33(4):989-93 PMID: 405929
  34. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  35. Mapping of mutations affecting synthesis of exocellular enzymes in Bacillus subtilis. Identity of the sacUh, amyB and pap mutations.
    Mol Gen Genet. 1976 Nov 17;148(3):281-5 PMID: 827683
  36. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.1.113-119.1986
Published
1986-04-00
Pages
113-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214565
Subset
IM
Databases
GENBANK
K03420, M12501
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/jb.166.1.113-119.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2