prtR enhances the mRNA level of the Bacillus subtilis extracellular proteases.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 3044-50

Tanaka T, Kawata M, Nagami Y, Uchiyama H

Abstract

Studies were performed on the prtR gene which enhances the production of the Bacillus subtilis extracellular proteases and levansucrase, but not the alpha-amylase, RNase, and alkaline phosphatase. To investigate the mode of action of prtR, the Escherichia coli bla gene was placed under the control of two promoters. One was the promoter of the alkaline protease gene (aprE), and the other was the promoter of B. subtilis dihydrofolate reductase gene (dfrA). Expression of the bla gene was enhanced by prtR only when the apr promoter was used. From these results, it was concluded that the apr promoter or its vicinity was the target of prtR and that prtR does not affect the process after transcription. The mRNA levels of aprE and nprE (the neutral protease gene) were significantly increased by prtR, but the half-life of the aprE mRNA was not affected. These results show that the prtR gene product enhances protease production by increasing the rate of transcription initiation.

MeSH Terms

Bacillus subtilis/enzymology,genetics Extracellular Space/enzymology Gene Expression Regulation Genes, Bacterial Genes, Regulator Peptide Hydrolases/genetics Promoter Regions, Genetic RNA, Messenger/genetics,metabolism Transcription Factors/genetics Transcription, Genetic

Chemicals

RNA, Messenger Transcription Factors Peptide Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tanaka T

Kawata M

Nagami Y

Uchiyama H

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37 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.7.3044-3050.1987

Published

1987-07-00

Pages

3044-50

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212346

Subset

External Links

PubMed source DOI: 10.1128/jb.169.7.3044-3050.1987 PMC Full Text View journal details

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prtR enhances the mRNA level of the Bacillus subtilis extracellular proteases.

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