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PMID: 3034860 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2579-90

Schnetz K, Toloczyki C, Rak B

Abstract

Wild-type Escherichia coli cells are unable to grow on beta-glucosides. Spontaneous mutants arise, however, which are able to utilize certain aromatic beta-glucosides such as salicin or arbutin as carbon sources, revealing the presence of a cryptic operon called bgl. Mutations activating the operon map within (or close to) the promoter region of the operon and are due to the transposition of an IS1 or IS5 insertion element into this region. This operon was reported to consist of three genes coding for a phospho-beta-glucosidase, a specific transport protein (enzyme IIBgl), and a positively regulating protein. We have defined the extent and location of three structural genes, bglC, bglS, and bglB, and have determined their DNA sequence. The amino acid sequences deduced from the open reading frames together with deletion and subcloning analyses suggest that the first gene, bglC, codes for the regulatory protein, the second, bglS, codes for the transport protein, and the third, bglB, for phospho-beta-glucosidase. A fourth gene may exist which codes for a product of unknown function. We discuss structural features of the DNA sequence which may bear on the regulation of the operon. Homologies to sequences preceding the gene for an excreted levansucrase of Bacillus subtilis, which are known to be involved in the regulation of this gene, and to sequences preceding the gene for an excreted beta-endoglucanase of B. subtilis, for which data pertaining to regulation are not yet available, suggest a close evolutionary relationship among the regulatory components of all three systems.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Glucosides/metabolism Glycosides/metabolism Membrane Proteins/genetics Operon Phosphoenolpyruvate Sugar Phosphotransferase System/genetics Promoter Regions, Genetic Protein Sorting Signals/genetics Repressor Proteins/genetics Sequence Homology, Nucleic Acid Solubility
Chemicals
Glucosides Glycosides Membrane Proteins Protein Sorting Signals Repressor Proteins Phosphoenolpyruvate Sugar Phosphotransferase System DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schnetz K
Toloczyki C
Rak B
References (44)
44 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2579-2590.1987
Published
1987-06-00
Pages
2579-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212127
Subset
IM
Databases
GENBANK
M16487
External Links
PubMed source DOI: 10.1128/jb.169.6.2579-2590.1987 PMC Full Text View journal details
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