Home LiteratureArticle Details
PMID: 3014438 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4881-97

Margolin W, Howe MM

Abstract

The C gene of bacteriophage Mu, required for transcription of the phage late genes, was localized by construction and analysis of a series of deleted derivatives of pKN50, a plasmid containing a 9.4 kb Mu DNA fragment which complements Mu C amber mutant phages for growth. One such deleted derivative, pWM10, containing only 0.5 kb of Mu DNA, complements C amber phages and transactivates the mom gene, one of the Mu late genes dependent on C for activation. The DNA sequence of the 0.5 kb fragment predicts a single long open reading frame coding for a 140 amino acid protein. Sequence analysis of DNA containing a C amber mutation located the base change to the second codon of this reading frame. Generation of a frameshift mutation by filling in a BglII site spanning codon 114 of this reading frame resulted in the loss of C complementation and transactivation activity. These results indicate that this open reading frame encodes the Mu C gene product. Comparison of the predicted amino acid sequence of the C protein with those of other transcriptional regulatory proteins revealed some similarity to a region highly conserved among bacterial sigma factors.

MeSH Terms
Amino Acid Sequence Bacteriophage mu/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Endonucleases Gene Expression Regulation Genes, Viral Genetic Complementation Test Single-Strand Specific DNA and RNA Endonucleases Transcription Factors/genetics Viral Proteins/genetics
Chemicals
DNA, Viral Transcription Factors Viral Proteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Margolin W
Howe M M
References (49)
49 references, click to expand
  1. Insertion of DNA carrying ribosomal protein genes of Escherichia coli into Charon vector phages.
    J Biol Chem. 1977 Oct 25;252(20):7344-54 PMID: 332692
  2. Isolation of mutations defining five new cistrons essential for development of bacteriophage Mu.
    Virology. 1979 Mar;93(2):303-19 PMID: 452408
  3. Correction and refinement of the genetic map of bacteriophage Mu.
    Virology. 1979 Mar;93(2):320-8 PMID: 452409
  4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  5. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  6. Mapping of the modification function of temperate phage Mu-1.
    Mol Gen Genet. 1980 Jan;177(2):351-3 PMID: 6445035
  7. Specificity of the bacteriophage Mu mom+ -controlled DNA modification.
    J Virol. 1980 Apr;34(1):277-9 PMID: 6445426
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. Cloning of DNA fragments of the right end of phage mu and location of the HindIII, SalI, PstI, and BamHI restriction sites on the genetic map of mu.
    Virology. 1980 Jul 30;104(2):418-25 PMID: 6249035
  10. Invertible DNA determines host specificity of bacteriophage mu.
    Nature. 1980 Jul 17;286(5770):218-22 PMID: 6250048
  11. In vitro synthesis of bacteriophage lysozyme.
    Nature. 1967 Aug 5;215(5101):588-91 PMID: 6050209
  12. Estimation of secondary structure in ribonucleic acids.
    Nature. 1971 Apr 9;230(5293):362-7 PMID: 4927725
  13. Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage.
    Virology. 1973 Jul;54(1):117-24 PMID: 4576742
  14. Prophage deletion mapping of bacteriophage Mu-1.
    Virology. 1973 Jul;54(1):93-101 PMID: 4351616
  15. Amino acid difference formula to help explain protein evolution.
    Science. 1974 Sep 6;185(4154):862-4 PMID: 4843792
  16. On the control of transcription of bacteriophage Mu.
    Mol Gen Genet. 1974;131(2):85-96 PMID: 4420740
  17. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  18. The DNA modification function of temperate phage Mu-1.
    Virology. 1976 Mar;70(1):17-27 PMID: 1258376
  19. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  20. Partial correlation of the genetic and physical maps of bacteriophage Mu.
    Virology. 1977 Apr;77(2):677-88 PMID: 855188
  21. Kinetics of Mu DNA synthesis.
    Mol Gen Genet. 1977 Mar 7;151(2):169-74 PMID: 876022
  22. Correlation of the genetic and physical maps in the central region of the bacteriophage Mu genome.
    Virology. 1980 Aug;105(1):185-95 PMID: 6251604
  23. Organization of transcriptional signals in plasmids pBR322 and pACYC184.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):167-71 PMID: 7017708
  24. Structural polypeptides and products of late genes of bacteriophage Mu: characterization and functional aspects.
    J Mol Biol. 1981 Jan 5;145(1):139-63 PMID: 6455529
  25. The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.
    Nucleic Acids Res. 1981 Jun 25;9(12):2889-903 PMID: 6269063
  26. Regulation of integration and replication functions of bacteriophage Mu.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:347-53 PMID: 6457724
  27. Ner, a cro-like function of bacteriophage Mu.
    Virology. 1982 Nov;123(1):19-28 PMID: 6293190
  28. Transcription initiation of Mu mom depends on methylation of the promoter region and a phage-coded transactivator.
    Nature. 1983 Jan 27;301(5898):344-7 PMID: 6218410
  29. The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
    Cell. 1983 Feb;32(2):335-49 PMID: 6186393
  30. Structure of a Bacillus subtilis bacteriophage SPO1 gene encoding RNA polymerase sigma factor.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1236-40 PMID: 6402778
  31. AvaII and BglI restriction maps of bacteriophage Mu.
    Virology. 1983 Apr 30;126(2):563-75 PMID: 6305002
  32. The early promoter of bacteriophage Mu: definition of the site of transcript initiation.
    Nucleic Acids Res. 1983 Aug 25;11(16):5483-95 PMID: 6310502
  33. Three promoters near the termini of IS10: pIN, pOUT, and pIII.
    Cell. 1983 Sep;34(2):673-82 PMID: 6311437
  34. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  35. Mapping of the Q-utilization site (qut) required for antitermination of late transcription in bacteriophage lambda.
    Gene. 1983 Dec;26(2-3):291-4 PMID: 6231216
  36. Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.
    Cell. 1984 Aug;38(1):175-82 PMID: 6088062
  37. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  38. A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coli.
    Nature. 1984 Nov 22-28;312(5992):376-8 PMID: 6438529
  39. Bacteriophage SPO1 genes 33 and 34. Location and primary structure of genes encoding regulatory subunits of Bacillus subtilis RNA polymerase.
    J Mol Biol. 1984 Dec 15;180(3):533-47 PMID: 6441846
  40. Positive control of transcription initiation in bacteria.
    Annu Rev Genet. 1984;18:173-206 PMID: 6084980
  41. A strong sequence homology exists between the major RNA polymerase sigma factors of Bacillus subtilis and Escherichia coli.
    J Biol Chem. 1985 Jun 25;260(12):7178-85 PMID: 2987246
  42. Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.
    EMBO J. 1985 Jan;4(1):257-64 PMID: 4018026
  43. Two functional domains conserved in major and alternate bacterial sigma factors.
    FEBS Lett. 1985 Jul 22;187(1):11-5 PMID: 3926534
  44. Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu.
    Virology. 1985 Jun;143(2):485-504 PMID: 3904174
  45. The nucleotide sequence of the nitrogen-regulation gene ntrA of Klebsiella pneumoniae and comparison with conserved features in bacterial RNA polymerase sigma factors.
    Nucleic Acids Res. 1985 Nov 11;13(21):7607-20 PMID: 2999700
  46. Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7525-9 PMID: 2999766
  47. Involvement of the invertible G segment in bacteriophage mu tail fiber biosynthesis.
    Virology. 1984 Apr 30;134(2):296-317 PMID: 6242484
  48. Regulation and expression of the bacteriophage mu mom gene: mapping of the transactivation (dad) function to the C region.
    Gene. 1985;39(1):71-6 PMID: 2934297
  49. Sensitive mutants of bacteriophage lambda.
    Virology. 1961 May;14:22-32 PMID: 13690195
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4881
Published
1986-06-25
Pages
4881-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311498
Subset
IM
Grants
NIAID NIH HHS · AI-12731 · United States
Databases
GENBANK
X03992
External Links
PubMed source DOI: 10.1093/nar/14.12.4881 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2