Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 182-91

Lew DJ, Decker T, Strehlow I, Darnell JE

Abstract

The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [IFN-alpha] or type II (IFN-gamma). The promoter of the GBP gene was cloned and found to contain an IFN-alpha-stimulated response element, which mediated the response of the GBP gene to IFN-alpha. On the basis of transfection experiments with recombinant plasmids, two different elements were delineated. Both were required to obtain the maximal response of the GBP gene to IFN-gamma: the IFN-alpha-stimulated response element and an overlapping element termed the IFN-gamma activation site. Different proteins that act on each element were investigated, and their possible involvement in IFN-gamma-induced transcriptional regulation is discussed.

MeSH Terms

Base Sequence Binding Sites Blotting, Southern Cloning, Molecular DNA Mutational Analysis DNA-Binding Proteins/physiology GTP-Binding Proteins/genetics Gene Expression Regulation/drug effects HeLa Cells Humans In Vitro Techniques Interferon Type I/pharmacology Interferon-gamma/pharmacology Molecular Sequence Data Multigene Family Nuclear Proteins/physiology Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Transcription, Genetic/drug effects

Chemicals

DNA-Binding Proteins Interferon Type I Nuclear Proteins Interferon-gamma GTP-Binding Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lew D J

Rockefeller University, New York, New York 10021-6399.

Decker T

Strehlow I

Darnell J E

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32 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.11.1.182-191.1991

Published

1991-01-00

Pages

182-91

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC359608

Subset

External Links

PubMed source DOI: 10.1128/mcb.11.1.182-191.1991 PMC Full Text View journal details

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Overlapping elements in the guanylate-binding protein gene promoter mediate transcriptional induction by alpha and gamma interferons.

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