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PMID: 2457496 Published · ppublish English Comparative Study Journal Article

Enhancer-like interferon responsive sequences of the human and murine (2'-5') oligoadenylate synthetase gene promoters.

The EMBO journal ·Vol. 7 ·No. 5 ·1988-05-00 ·Pages 1411-9

Cohen B, Peretz D, Vaiman D, Benech P, Chebath J

Abstract

The human (2'-5') oligo(A) synthetase gene contains two independent cis-acting DNA elements, A and B, which act as transcriptional enhancers. Element A alone is not activated by IFN treatment. Element B alone confers IFN-inducibility to the herpes tk promoter. Two murine (2'-5') oligo(A) synthetase genes were isolated and their promoter sequences show high conservation of element A and B. A synthetic oligonucleotide, containing 16 bp of the human element B, or 14 bp of the homologue murine element B, was linked to a TK-CAT construct. These oligonucleotides were shown to be sufficient to activate the TK promoter in the presence of IFN. When multiple repeats of the interferon-responsive sequence (E-IRS) were cloned in 5' of the TK promoter, the activation ratio was increased. In vitro, specific binding of nuclear protein(s) is observed to the radiolabelled synthetic human E-IRS. This binding is competed by the addition of cold synthetic mouse E-IRS or fragments of genomic DNA containing the E-IRS.

MeSH Terms
2',5'-Oligoadenylate Synthetase/genetics Animals Base Sequence DNA/genetics Enhancer Elements, Genetic/drug effects Histocompatibility Antigens/genetics Humans Interferons/pharmacology Mice Molecular Sequence Data Multigene Family Promoter Regions, Genetic/drug effects Protein Binding Sequence Homology, Nucleic Acid
Chemicals
Histocompatibility Antigens DNA Interferons 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cohen B
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
Peretz D
Vaiman D
Benech P
Chebath J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-05-00
Pages
1411-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC458391
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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