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PMID: 3336368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The cell-specific enhancer of the mouse transthyretin (prealbumin) gene binds a common factor at one site and a liver-specific factor(s) at two other sites.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 81-90

Costa RH, Lai E, Grayson DR, Darnell JE

Abstract

We previously defined two distinct cell-specific DNA elements controlling the transient expression of the transthyretin gene in Hep G2 (human hepatoma) cells: a proximal promoter region (-202 base pairs [bp] to the cap site), and a far-upstream cell-specific enhancer located between 1.6 and 2.15 kilobases (kb) 5' of the cap site (R. H. Costa, E. Lai, and J. E. Darnell, Jr., Mol. Cell. Biol. 6:4697-4708, 1986). In this report, we located the effective transthyretin enhancer element within a 100-bp region between 1.96 and 1.86 kb 5' to the mRNA cap site. In Hep G2 nuclear extracts, three protein-binding sites within this minimal enhancer element were identified by gel mobility and methylation protection experiments. Each binding site was required for full enhancer activity in Hep G2 transient expression assays. Competition experiments in protein-binding assays suggested that two of the three sites were recognized by a similar factor and that the protein interaction with the third site was different. The nuclear protein(s) which bound to the two homologous sites was found mainly or only in cells of hepatic origin, suggesting an involvement of this region in the cell-specific function of this enhancer. The nuclear protein(s) recognizing the third enhancer region was also found in HeLa and spleen cells.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Humans Liver/physiology Mice Molecular Sequence Data Nuclear Proteins/metabolism Oligonucleotides/metabolism Prealbumin/genetics Regulatory Sequences, Nucleic Acid Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Nuclear Proteins Oligonucleotides Prealbumin Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Costa R H
Rockefeller University, New York, New York 10021.
Lai E
Grayson D R
Darnell J E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.81-90.1988
Published
1988-01-00
Pages
81-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363083
Subset
IM
Grants
NIAID NIH HHS · AI 07233-11 · United States
NCI NIH HHS · CA 160006-14 · United States
NCI NIH HHS · CA 18213-11 · United States
Databases
GENBANK
J03438, J03829, M19523
External Links
PubMed source DOI: 10.1128/mcb.8.1.81-90.1988 PMC Full Text View journal details
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