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PMID: 3865198 Published · ppublish English Journal Article

Lens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 7815-9

Overbeek PA, Chepelinsky AB, Khillan JS, Piatigorsky J, Westphal H

Abstract

Two lines of transgenic mice with one to two copies of a DNA fragment containing nucleotides -364 to +45 of the murine alpha A-crystallin gene linked to the bacterial chloramphenicol acetyltransferase (CAT) gene expressed the CAT gene only in their eye lenses. Both CAT activity and alpha A-crystallin were first detected in eyes at approximately 12.5 days of embryonic development, suggesting that the alpha A-CAT fusion gene and the endogenous alpha A-crystallin gene are co-regulated during lens development in the transgenic mice. These experiments show that the murine alpha A-crystallin gene contains a short, cis-acting, tissue-specific regulatory sequence at its 5' end that can target the expression of the bacterial CAT gene, and probably foreign eukaryotic genes, specifically to the ocular lens.

MeSH Terms
Acetyltransferases/genetics Animals Chloramphenicol O-Acetyltransferase Crystallins/genetics DNA, Recombinant Gene Expression Regulation Genetic Engineering Lens, Crystalline/embryology,physiology Mice Promoter Regions, Genetic RNA, Messenger/genetics
Chemicals
Crystallins DNA, Recombinant RNA, Messenger Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Overbeek P A
Chepelinsky A B
Khillan J S
Piatigorsky J
Westphal H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.7815
Published
1985-12-00
Pages
7815-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390860
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.23.7815 PMC Full Text View journal details
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