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PMID: 2540952 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two different virus-inducible elements are required for human beta-interferon gene regulation.

The EMBO journal ·Vol. 8 ·No. 1 ·1989-01-00 ·Pages 101-10

Fan CM, Maniatis T

Abstract

We show that the human beta-interferon gene promoter contains two different virus-inducible regulatory elements, PRDI and PRDII (positive regulatory domains I and II). A single copy of either element alone has no discernible transcriptional activity in mouse fibroblasts. However, multiple copies of either element function as a constitutive or virus-inducible transcription element depending on the cell line in which the sequence was tested. These results in conjunction with previous studies suggest that virus induction of the human beta-interferon gene is achieved through cooperative interactions between two entirely distinct virus-inducible elements. Comparison of the properties of these elements reveals that multiple copies of PRDI, but not PRDII, can be activated by three different inducers, beta-IFN, gamma-IFN and virus. These results suggest that the pathways of virus and interferon induction may share at least one common regulatory component.

MeSH Terms
Animals Base Sequence Enhancer Elements, Genetic Gene Expression Regulation/drug effects Genes, Viral Humans Interferon Type I/biosynthesis,genetics,pharmacology Interferon-gamma/pharmacology L Cells Mice Molecular Sequence Data Parainfluenza Virus 1, Human/physiology Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Simian virus 40/genetics Species Specificity
Chemicals
Interferon Type I Interferon-gamma
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fan C M
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Maniatis T
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-01-00
Pages
101-10
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400777
Subset
IM
Grants
PHS HHS · A1-20642 · United States
External Links
PubMed source PMC Full Text View journal details
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