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PMID: 2974922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3065-79

Hug H, Costas M, Staeheli P, Aebi M, Weissmann C

Abstract

Specific resistance of Mx+ mice to influenza virus is due to the interferon (IFN)-induced protein Mx. The Mx gene consists of 14 exons that are spread over at least 55 kilobase pairs of DNA. Surprisingly, the Mx gene promoter is induced as efficiently by Newcastle disease virus as it is by IFN. The 5' boundary of the region required for maximal induction by both IFN and Newcastle disease virus is located about 140 base pairs upstream of the cap site. This region contains five elements of the type GAAANN, which occurs in all IFN- and virus-inducible promoters. The consensus sequence purine-GAAAN(N/-)GAAA(C/G)-pyrimidine is found in all IFN-inducible promoters.

MeSH Terms
Amino Acid Sequence Animals Antiviral Agents Bacteriophage lambda/genetics Base Sequence Cell Line Cloning, Molecular Cosmids GTP-Binding Proteins Genes Interferon Type I/physiology Mice Mice, Inbred BALB C Molecular Sequence Data Myxovirus Resistance Proteins Newcastle disease virus/genetics Promoter Regions, Genetic Proteins/genetics RNA, Messenger/genetics Recombinant Proteins/pharmacology Transcription, Genetic Transfection
Chemicals
Antiviral Agents Interferon Type I Mx1 protein, mouse Myxovirus Resistance Proteins Proteins RNA, Messenger Recombinant Proteins GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hug H
Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Costas M
Staeheli P
Aebi M
Weissmann C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3065-3079.1988
Published
1988-08-00
Pages
3065-79
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363533
Subset
IM
Databases
GENBANK
M21104, M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116, M21117
External Links
PubMed source DOI: 10.1128/mcb.8.8.3065-3079.1988 PMC Full Text View journal details
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